Figure 24.7 shows the analysis of a polypeptide hydrolysate by ion-exchange chromatography.
FIGURE 24.7 Analysis of a mixture of amino acids by ion-exchange chromatography using Amberlite IR-120, a sulfonated polystyrene resin. The resin contains phenyl-SO3-Na+ groups. The amino acid mixture is applied to the column at low pH (3.25), conditions under which the acidic amino acids (Asp, Glu) are weakly bound to the resin and the basic amino acids (Lys, His, Arg) are tightly bound. Sodium citrate buffers of two different concentrations and three different values of pH are used to elute the amino acids from the column. Cysteine is determined as cystine, Cy-S-S-Cys, the disulfide of cysteine.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
Stoned Mountain was very rocky today. Somehow I didn't break anything lol. Had a great time with good friends. Awesome day #goodday #scx10 #scx10iii #axial #deathstroke #proline #duramax #420 #zero #stonemountain (at Stone Mountain Walk-Up Trail) https://www.instagram.com/p/ColW8tzMJEA/?igshid=NGJjMDIxMWI=
To build a strong skeleton, osteoblasts, the cells that produce bone, need plenty of amino acids, the building blocks of proteins. Many proteins involved in bone formation are especially rich in a specific amino acid, proline, so getting enough of it during development is critical for cells to first differentiate into osteoblasts, then make bone. Recent research in mice identified a major route for developing osteoblasts to acquire proline, through a transporter known as SLC38A2. Knocking down the gene for SLC38A2 affected cells’ ability to make key proteins, with consequences for skeletal development: fifteen days after conception, mouse embryos have a recognisable skeleton, including mineralised bone (pictured in pink, with cartilage in blue), but this is substantially reduced in mice lacking SLC38A2. As proline is so crucial to bone development, exploring its role in adulthood might suggest whether proline supplements could also help strengthen bones weakened by age or disease.
Written by Emmanuelle Briolat
Image from work by Leyao Shen and colleagues
Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC, USA
Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in eLife, March 2022
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Table 24.2 gives pKa values for each ionisable group of the 20 protein-derived amino acids. (...) The isoelectric points for common amino acids are given in table 24.2.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
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