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Figure 24.7 shows the analysis of a polypeptide hydrolysate by ion-exchange chromatography.
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FIGURE 24.7 Analysis of a mixture of amino acids by ion-exchange chromatography using Amberlite IR-120, a sulfonated polystyrene resin. The resin contains phenyl-SO3-Na+ groups. The amino acid mixture is applied to the column at low pH (3.25), conditions under which the acidic amino acids (Asp, Glu) are weakly bound to the resin and the basic amino acids (Lys, His, Arg) are tightly bound. Sodium citrate buffers of two different concentrations and three different values of pH are used to elute the amino acids from the column. Cysteine is determined as cystine, Cy-S-S-Cys, the disulfide of cysteine.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
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Aminoacid Swag Tournament Round 2
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galgorithm · 1 year
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Kingair 350 G1000 v Proline 21 v Proline Fusion cockpits
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bobhollo · 1 year
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Stoned Mountain was very rocky today. Somehow I didn't break anything lol. Had a great time with good friends. Awesome day #goodday #scx10 #scx10iii #axial #deathstroke #proline #duramax #420 #zero #stonemountain (at Stone Mountain Walk-Up Trail) https://www.instagram.com/p/ColW8tzMJEA/?igshid=NGJjMDIxMWI=
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bpod-bpod · 2 years
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Packed with Proline
To build a strong skeleton, osteoblasts, the cells that produce bone, need plenty of amino acids, the building blocks of proteins. Many proteins involved in bone formation are especially rich in a specific amino acid, proline, so getting enough of it during development is critical for cells to first differentiate into osteoblasts, then make bone. Recent research in mice identified a major route for developing osteoblasts to acquire proline, through a transporter known as SLC38A2. Knocking down the gene for SLC38A2 affected cells’ ability to make key proteins, with consequences for skeletal development: fifteen days after conception, mouse embryos have a recognisable skeleton, including mineralised bone (pictured in pink, with cartilage in blue), but this is substantially reduced in mice lacking SLC38A2. As proline is so crucial to bone development, exploring its role in adulthood might suggest whether proline supplements could also help strengthen bones weakened by age or disease.
Written by Emmanuelle Briolat
Image from work by Leyao Shen and colleagues
Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC, USA
Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in eLife, March 2022
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milujeme-baseball · 6 months
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Martin Pernička stráví zimu hraním baseballu na Arubě
Karibský ostrov Aruba se kromě krásných pláží může již druhým rokem pyšnit také zimní baseballovou ligou, která nese název Proline Baseball. Letos tato ambiciózní soutěž ale ponese hned několik českých stop, tou nejvýraznější je angažmá Martina Perničky, jenž si v ní zahraje jako první český baseballista vůbec. Pro našince je tato soutěž absolutní neznámou a tak jsme přes Martina zjišťovali,…
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iventilation · 11 months
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Range Hoods vs. Over-the-Range Microwaves: Which Should You Choose?
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When it comes to the kitchen, two popular options for enhancing functionality and saving space are range hoods and over-the-range microwaves. Both serve distinct purposes and offer unique benefits. In this article, we will compare these options based on safety considerations, maintenance and durability, energy efficiency, detailed cost breakdown, case studies or customer reviews, and expert recommendations. By considering these factors, you can make an informed decision on which option suits your needs best.
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rockncrawlers · 1 year
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【PROLINEボディ紹介】1/6 1956 Ford F-100 カット済み 〜 PRO361017 – X-MAXX ■WB480mm
【PROLINEボディ紹介】1/6 1956 Ford F-100 カット済み 〜 PRO361017 – X-MAXX ■WB480mm
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machinedalal · 2 years
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Heidelberg - #Stahlfolder Ti 40/3 Proline https://dl.machinedalal.com/nFWU
Manufacturer: #Heidelberg Year: 2011 Machine Availability: Immediately from stock Price: On Request Location: Germany
#Ti403 #Proline #postpress #FoldingMachine #machinedalal
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cytgen · 2 years
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sunplix-cmh-light · 2 years
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Posted @withregram • @gucci_green_ The girls are finally hitting cruise control, its been a rocky start my fault trying to rush things 🙃 but this way of growing is all new to me (gotta try and keep up with the game) I will veg for another week than flip. 🇦🇺😈💥 So these are hubba runtz by @runtz Under g2 led by @sunplix_cmh_led Sitting in the 3 gallon pots system by @thebucketcompany Getting fed @athena.ag #proline with a ec of 2 💚💚 #wegrowtrees #AthenaAg #ausgrown #thebucketcompany #guccigreen #hightimes #selfmedication #growyourown #led #mountainair #worksmarternotharder #alwayslearning #420 #710 #notimetohate #itsjustaplant #notharminganyone #runtz #bubbakush #tobusygrinding #trusttheprocess #sunplix #led #growlight #sunplixled #sunplixg2 https://www.instagram.com/p/CgfM27_OomP/?igshid=NGJjMDIxMWI=
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Table 24.2 gives pKa values for each ionisable group of the 20 protein-derived amino acids. (...) The isoelectric points for common amino acids are given in table 24.2.
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"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
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Rip proline. You will be remembered as my favourite trouble-making little shit.
Press F to pay respects.😔
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hghydroponics · 2 years
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#Athena Pro Line now back in stock @hghydroponics #stockgoingfast 📲 Order online 24/7 or drop in 9-5 #lincoln #lincolnshire #athena #athenanutrients #proline #plantnutrition #growers #hydroponics #gardening #gardener (at Lincoln, Lincolnshire) https://www.instagram.com/p/Cb_FwPHtmvI/?igshid=NGJjMDIxMWI=
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ancient-healer · 2 years
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The summary would be this.
Proline comes from the meat of well cared for animals.
Proline can cause depression.
But not always.
Only in those with damaged intestines. (Vaccines and antibiotics cause gut damage.)
In genetically modified flies proline was unable to reach the brain.
So what's the take away here? 🙄
Brainwashing much? The herd is falling for it all.
Pureblood and proud.
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notfye · 7 months
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not my sudden obsession with scurvy helping me answer a question in histology
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