Figure 24.7 shows the analysis of a polypeptide hydrolysate by ion-exchange chromatography.
FIGURE 24.7 Analysis of a mixture of amino acids by ion-exchange chromatography using Amberlite IR-120, a sulfonated polystyrene resin. The resin contains phenyl-SO3-Na+ groups. The amino acid mixture is applied to the column at low pH (3.25), conditions under which the acidic amino acids (Asp, Glu) are weakly bound to the resin and the basic amino acids (Lys, His, Arg) are tightly bound. Sodium citrate buffers of two different concentrations and three different values of pH are used to elute the amino acids from the column. Cysteine is determined as cystine, Cy-S-S-Cys, the disulfide of cysteine.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
Stoned Mountain was very rocky today. Somehow I didn't break anything lol. Had a great time with good friends. Awesome day #goodday #scx10 #scx10iii #axial #deathstroke #proline #duramax #420 #zero #stonemountain (at Stone Mountain Walk-Up Trail) https://www.instagram.com/p/ColW8tzMJEA/?igshid=NGJjMDIxMWI=
To build a strong skeleton, osteoblasts, the cells that produce bone, need plenty of amino acids, the building blocks of proteins. Many proteins involved in bone formation are especially rich in a specific amino acid, proline, so getting enough of it during development is critical for cells to first differentiate into osteoblasts, then make bone. Recent research in mice identified a major route for developing osteoblasts to acquire proline, through a transporter known as SLC38A2. Knocking down the gene for SLC38A2 affected cells’ ability to make key proteins, with consequences for skeletal development: fifteen days after conception, mouse embryos have a recognisable skeleton, including mineralised bone (pictured in pink, with cartilage in blue), but this is substantially reduced in mice lacking SLC38A2. As proline is so crucial to bone development, exploring its role in adulthood might suggest whether proline supplements could also help strengthen bones weakened by age or disease.
Written by Emmanuelle Briolat
Image from work by Leyao Shen and colleagues
Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, NC, USA
Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in eLife, March 2022
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Martin Pernička stráví zimu hraním baseballu na Arubě
Karibský ostrov Aruba se kromě krásných pláží může již druhým rokem pyšnit také zimní baseballovou ligou, která nese název Proline Baseball. Letos tato ambiciózní soutěž ale ponese hned několik českých stop, tou nejvýraznější je angažmá Martina Perničky, jenž si v ní zahraje jako první český baseballista vůbec.
Pro našince je tato soutěž absolutní neznámou a tak jsme přes Martina zjišťovali,…
Range Hoods vs. Over-the-Range Microwaves: Which Should You Choose?
When it comes to the kitchen, two popular options for enhancing functionality and saving space are range hoods and over-the-range microwaves. Both serve distinct purposes and offer unique benefits. In this article, we will compare these options based on safety considerations, maintenance and durability, energy efficiency, detailed cost breakdown, case studies or customer reviews, and expert recommendations. By considering these factors, you can make an informed decision on which option suits your needs best.
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By 1964, all 64 codons had been deciphered (table 25.4). (...) Several features of the genetic code are evident from a study of table 25.4.
Only 61 triplets code for amino acids. The remaining three (UAA, UAG and UGA) are signals for chain termination; they signal to the protein-synthesizing machinery of the cell that the primary sequence of the protein is complete. The three chain termination triplets are indicated in the table by 'Stop'.
The code is degenerate, which means that several amino acids are coded for by more than one triplet. Only methionine and tryptophan are coded for by just one triplet. Leucine, serine and arginine are coded for by six triplets, and the remaining amino acids are coded for by two, three or four triplets.
For the 15 amino acids coded for by two, three or four triplets, it is only the third letter of the code that varies. For example, glycine is coded for by the triplets GGA, GGG, GGC and GGU.
There is no ambiguity in the code, meaning that reach triplet codes for only one amino acid.
AUG is the initiation signal, and it is also the codon for the amino acid methionine. In all protein synthesis, the first amino acid placed is always methionine.
"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.
#Athena Pro Line now back in stock @hghydroponics #stockgoingfast 📲 Order online 24/7 or drop in 9-5 #lincoln #lincolnshire #athena #athenanutrients #proline #plantnutrition #growers #hydroponics #gardening #gardener (at Lincoln, Lincolnshire) https://www.instagram.com/p/Cb_FwPHtmvI/?igshid=NGJjMDIxMWI=