A new approach to producing artificial cartilage with the help of 3D printing
Is it possible to grow tissue in the laboratory, for example to replace injured cartilage? At TU Wien (Vienna), an important step has now been taken toward creating replacement tissue in the lab—using a technique that differs significantly from other methods used around the world. The study is published in Acta Biomaterialia.
A special high-resolution 3D printing process is used to create tiny, porous spheres made of biocompatible and degradable plastic, which are then colonized with cells. These spheroids can then be arranged in any geometry, and the cells of the different units combine seamlessly to form a uniform, living tissue. Cartilage tissue, with which the concept has now been demonstrated at TU Wien, was previously considered particularly challenging in this respect.
Tiny spherical cages as a scaffold for the cells
"Cultivating cartilage cells from stem cells is not the biggest challenge. The main problem is that you usually have little control over the shape of the resulting tissue," says Oliver Kopinski-Grünwald from the Institute of Materials Science and Technology at TU Wien, one of the authors of the current study. "This is also due to the fact that such stem cell clumps change their shape over time and often shrink."
Just a reminder that the same genetics that give the folded ears of Scottish Fold cats applies to the rest of the cartilage in their bodies and leads to early onset and increasingly severe arthritis.
ok i'm mainly posting it to scare myself into staying away from tumblr but. the bebes. did these a while ago to test some things out so they're. super inconsistent and not 100% up-to-date anymore but i still kinda like them
It’s tough, it’s rubbery, it’s your cartilage. Your joints contain two types: articular cartilage, which covers the ends of your bones, and growth-plate cartilage, which forms the ends of long bones. Both comprise cells called chondrocytes. How these types of cartilage develop isn’t clear. Researchers now investigate in mice genetically engineered with fluorescently-tagged NFATc1 – a protein which when inactivated interferes with cartilage development. Using fluorescent microscopy of developing mouse knee joints, they found NFATc1-containing cells (progenitors) matured into articular chondrocytes but not growth-plate chondrocytes. NFATc1-containing progenitors also matured into cells that contribute to the joint lining, ligaments and developing kneecap (pictured, green). Analysing the RNA of articular chondrocytes revealed that NFATc1 levels dropped as the cells matured. Reducing NFATc1 levels in progenitors triggered articular cartilage formation while increasing levels blocked this from happening. NFATc1 is therefore vital for articular — but not growth-plate — cartilage development.
Written by Lux Fatimathas
Image from work by Fan Zhang and Yuanyuan Wang, and colleagues
Xuanwu Hospital, Capital Medical University, China; National Clinical Research Center for Geriatric Diseases, China
Image originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in eLife, February 2023
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A little surgery I’ve gone through which I’d like to share with everyone. But, 1st and foremost, I wanna thank Allah The Al-Mighty for giving me the strength and blessing me with good health and waking me up from my surgery with good healing process. Alhamdulillah.
Thank you all at Columbia Asia Hospital especially Dr. Azfar for doing a great surgery on my knee. At the time of writing this, I am…
hey my cartilage piercing is infected. any of you guys have tips on how to bring it back to health and/or how to take better care of it once it’s back to normal?
for background on the situation, i’ve been using a saline spray on it once a day after i shower but i’m gonna step it up to be 3 times a day. currently using an ice pack because the area is hot but i’ve also read that using a warm compress is advised.