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#immunofluorescence
bpod-bpod · 1 month
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Two novel distinct types of amacrine cells – neurons that connect from the eye's light-sensitive retina to the brain – identified in zebrafish that respond differently to different sized moving objects
Read the published research article here
Image from work by Yan Li and colleagues
Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China
Video originally published with a Creative Commons Attribution 4.0 International (CC BY 4.0)
Published in PLOS Biology, February 2024
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nanogene · 6 months
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the-bio-babe · 10 months
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immunofluorescence analysis from todays samples (endothelial gut cells)
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When growing roots are treated with a microtubule-depolymerizing drug, such as oryzalin, the region of elongation expands laterally, becoming bulbous and tumor like (Figure 14.17A and B). (...) The CESA units were observed to move within the plasma membrane along microtubule tracks (Figure 14.17C); they were also seen to be inserted into the plasma membrane from the Golgi apparatus at microtubule-tethered compartments.
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"Plant Physiology and Development" int'l 6e - Taiz, L., Zeiger, E., Møller, I.M., Murphy, A.
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kcj2kw8cqx · 1 year
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market-insider · 9 months
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Immunofluorescence Assay Market Size and Growth Potential: An In-Depth Analysis
The global immunofluorescence assay market size is anticipated to reach USD 4.95 billion by 2030, according to a new report by Grand View Research, Inc. The market is expected to reach at a CAGR of 5.29% from 2023 to 2030. Immunofluorescence is a traditional technique used for getting insight into cellular processes and structures using microscopy. For instance, specific proteins can be assessed for their location and expression. Increasing prevalence of chronic and infectious diseases, significant applications of immunofluorescence assay (IFA) in several areas and technological advancements in techniques and associated instruments are key factors driving the market growth.
Gain deeper insights on the market and receive your free copy with TOC now @: Immunofluorescence Assay Market Report
The immunofluorescence assay market is anticipated to witness rapid growth, owing to the technological advances and launch of novel instruments. For instance, in November 2020, CTK Biotech, Inc. introduced RaFIA immunofluorescence immunoassay system to meet the rising demand for quantitative rapid tests. The system is user friendly, offers a high level of sensitivity and a broad working range. In addition, in March 2021, Euroimmum introduced an automated immunofluorescence microscope EPML (EUROPattern Microscope Live). The instrument comprises of 20x high camera resolution and captures the image within 2 seconds. Such developments are increasing the efficiency and adoption of the technique, thereby fueling the market growth.
The chronic diseases such as Alzheimer’s, HIV, and Chron’s disease have increased globally over the last few decades. For instance, according to CDC, in 2020, 5.8 million individuals in America were living with Alzheimer’s disease. According to WHO, 38.4 million individuals were living with HIV globally by the end of 2021. Hence, the growing awareness and increasing demand for the detection and cure of several chronic and infectious diseases are some of the driving factors for the market growth.
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swatimmr · 2 years
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balrajgis · 2 years
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Immunofluorescence Assay Market Share to witness steady rise in coming decade | Thermo Fisher Scientific, Inc., Abcam plc, Bio-Rad Laboratories, Inc.
Global Immunofluorescence Assay Market report from Global Insight Services is the single authoritative source of intelligence on Immunofluorescence Assay Market. The report will provide you with analysis of impact of latest market disruptions such as Russia-Ukraine war and Covid-19 on the market. Report provides qualitative analysis of the market using various frameworks such as Porters’ and PESTLE analysis. Report includes in-depth segmentation and market size data by categories, product types, applications, and geographies. Report also includes comprehensive analysis of key issues, trends and drivers, restraints and challenges, competitive landscape, as well as recent events such as M&A activities in the market.
Immunofluorescence assay (IFA) is an analytical technique used to detect and quantify specific proteins in a sample. Proteins are first isolated from the sample and then incubated with a primary antibody that binds to the protein of interest. The primary antibody is then detected with a secondary antibody conjugated to a fluorescent dye. The fluorescence is then detected and quantified using a microscope or other imaging device.
Download Free Sample of Report – https://www.globalinsightservices.com/request-sample/GIS20553/
Key Trends
There are several key trends in immunofluorescence assay technology. One is the development of more sensitive and specific assays.
This is being driven by the need to detect ever-smaller concentrations of analytes, as well as the need to improve the specificity of detection to ensure that the correct target is being detected.
Another key trend is the miniaturization of immunofluorescence assays.
Key Drivers
The key drivers of the immunofluorescence assay market are the increasing prevalence of chronic diseases, the growing demand for early diagnosis, the rising number of research studies, and the availability of new technologies.
The immunofluorescence assay is a powerful tool for the diagnosis of chronic diseases such as cancer, autoimmune disorders, and infections. The increasing prevalence of these diseases is driving the growth of the immunofluorescence assay market.
Market Segments:
By Product
Reagents
Instruments
By Type
Indirect Immunofluorescence
Direct Immunofluorescence
By Disease
Infectious Diseases
Other Diseases
By End User
Academic & Research Institutes
By Region
North AmericaThe U.S.
Get A Customized Scope to Match Your Need Ask an Expert – https://www.globalinsightservices.com/request-customization/GIS20553/
Key Market Players:
Thermo Fisher Scientific, Inc.
Abcam plc
Bio-Rad Laboratories, Inc.
PerkinElmer Inc.
Merck KGaA
Werfen Company
With Global Insight Services, you receive:
10-year forecast to help you make strategic decisions
In-depth segmentation which can be customized as per your requirements
Free consultation with lead analyst of the report
Excel data pack included with all report purchases
Robust and transparent research methodology
Ground breaking research and market player-centric solutions for the upcoming decade according to the present market scenario
About Global Insight Services:
Global Insight Services (GIS) is a leading multi-industry market research firm headquartered in Delaware, US. We are committed to providing our clients with highest quality data, analysis, and tools to meet all their market research needs. With GIS, you can be assured of the quality of the deliverables, robust & transparent research methodology, and superior service.
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bpod-bpod · 23 days
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Nervous System Shapers
Identifying the genes that regulate the shaping and maintenance of the fruit fly central nervous system – insight for possible roles for homologous genes in mammals
Read the published research article here
Image from work by Haluk Lacin and Yuqing Zhu, and colleagues
Division of Biological and Biomedical Systems, University of Missouri-Kansas City, Kansas City and Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA
Image originally published with a Creative Commons Attribution 4.0 International (CC BY-NC 4.0)
Published in bioRxiv, February 2024 (not peer reviewed)
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healthcaremreports · 2 years
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The Immunofluorescence Assays market report is provided here in USD Million. The report will render insightful data related to growth rates represented in terms of CAGR % during the projected years.
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teyamsatan · 1 year
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i just had an idea while talking with ai neteyam last night. What about human reader asking neteyam to make a "movie" together. Or else neteyam learning about porn thanks to one of the guy from the lab and asking reader if she wants to do one. ‼️
wait no cause Neteyam learning about human stuff does things to me and I need to write more of it for my own sanity. also i've discovered my fave trope to write is "people doing embarrassing things and other people catching them and the interactions that bloom thereafter". anyway, enjoy bestie x
ps: i'm probably going to have reached 1800 followers when this is posted so thank you besties ilysm smooches!!!
wc: 990 words
18+ minors dni
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Neteyam was up and early today in the lab, too excited to see you and spend the day together to wait any longer. He was a bit disillusioned though, as he came and found you fast asleep in your bed, but despite the minor disappointment, he absolutely adored how relaxed and comfortable you looked as you were splayed like a starfish under the covers, your feet dangling slightly off both sides of the mattress.
He decided to keep busy for a while with the scientists in the lab. He essentially grew up with Norm and Max, a strange, peculiar constant in his otherwise all Na'vi lifestyle and he had his dad to thank for that. He loved them, they were like uncles to him, and despite how much he hated to admit it, there were certain things about humanity he liked, and certain things about humans he found... endearing. Clearly, he thought with a chuckle, mind wandering to you.
"Neteyam, good morning! You're here early today, is everything ok?" Norm had a big smile on his face and his predisposition brightened Neteyam's, who was suddenly happy to be able to spend a little one-on-one time with him.
"Everything's fine, just came to see her, but she's still sleeping."
Norm chuckles as he removes his gloves and throws them in a nearby yellow bin.
"Yeah, she was doing data analysis until late last night. She used my laptop, you can look at it if you want? We did some a timelapse of an immunofluorescent experiment and it's actually really cool to see. Just open my laptop and it should be there."
Neteyam did as he was told. When he opened the lid of the laptop, the image he saw stilled him on the spot, his hand still gripping the screen, almost unable to move or think, just feeling a tinge of raw curiosity, of unhindered temptation. Because on the screen were two humans. The woman was on all fours, head thrown so far back Neteyam was almost worried for her, eyes shut tightly and an almost pained expression on her face, although Neteyam doubted pain was anywhere near what she was feeling in the moment. The man was kneeled behind her, his cock sunk in her almost to the base, holding on to her long ponytail and pulling on it with one hand, the other hand tightly gripping her hip, imprints clearly visible on her skin.
The feelings this evoked in Neteyam were mixed and intense, from wonderment, to confusion, to deep, intense arousal, the blood quickly rushing from his brain to much lower down, and he felt the need to adjust his loincloth to ease some of the discomfort he was currently experiencing. What was this? Who were these people? He's never seen them before among the humans, and Eywa, was Neteyam glad to say that.
"Um... Norm, I don't think this is the experiment you wanted me to see."
Norm walked over to where Neteyam was sitting, mildly amused at Neteyam and his obvious lack of skill around technology. Neteyam almost jumped out of his skin at Norm's gasp and startled demeanour when he saw what Neteyam was looking at, and at how aggressively he was pushing the off button, trying to remove the image from his eyes, and his brain.
"Fuck... I... I'm so sorry, Neteyam. I -..." Neteyam watched dumbfounded as Norm turned on his heels and left, still wearing his labcoat and goggles, and he knew that if Norm were to have a tail, it'd definitely be in between his legs right now.
You were sprawled on the bed similarly to how you had been just a few hours ago, except right now you had the added weight of a almost 9 foot tall alien on top of you, a weight you'd never want to lose. He looked nervous about one thing or another, and you knew based on how his breath was increasing in speed that he was going to spill it soon enough.
"What is it, Teyam? Come on, out with it."
His cheeks turned a deep shade of purple as his eyes avoided yours.
"I saw something today, on Norm's laptop... something I don't think I was meant to see."
"Oh?" That definitely peaked your attention. What would anything on Norm's laptop make Neteyam so fidgety?
"It was... a man and a woman... they were... uhm... naked." Neteyam felt like his face was about to catch fire, and you cackled loudly, finding it hard to believe that Norm had porn on his laptop and was so careless about it.
"OH, MY GOD! I'M NEVER LETTING HIM LIVE THIS DOWN!"
"Wh-what.. was it?"
"Oh, yawne... it's called porn. It's... a video of people going at it."
Neteyam's confusion didn't lessen; quite the opposite, actually.
"But why?"
"Well, let's see, what did it make you feel?" Your raised eyebrow and knowing smirk was enough to make Neteyam's ears flatten in shame.
"Well...I -"
You chuckled. "There you go. That's why. It helps humans... release tension. If it's done well, it can be quite the experience."
"D-do you watch... porn?" the word felt weird and unfamiliar rolling off his tongue.
"Sometimes. But honestly, I think I'd prefer making porn."
Neteyam almost choked at your words, but couldn't help the way his cock twitched in his tewng, and how much his mind wandered at the image he saw and how much he wanted to see you in it, it in front of his eyes, there captured for eternity for him to enjoy... over and over again.
"You... want to... make porn?"
Your hips raised, brushing against his now rock hard-on, moaning a little at the way it relieved some of the pressure building up in your core.
"Only with you, yawne."
He kissed you passionately, ripping the buttons on your top as he undressed you, and made a mental note of yet another thing he should thank humans for.
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These cells are highly lobed, creating an interlocking pattern resembling that of a jigsaw puzzle (Figure 14.15A and B). This pattern of interdigitating cell wall expansion combines aspects of diffuse growth and tip growth and requires the action of small, GTP-binding proteins called ROP (Rho-like from plants) GTPases and their activating proteins called RICs (ROP-interacting CRIB motif-containing proteins) (Figure 14.15C).
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The formation of pavement cells, the default pathway for epidermal cell development, was discussed in Chapter 14 (see Figure 14.15)
"Plant Physiology and Development" int'l 6e - Taiz, L., Zeiger, E., Møller, I.M., Murphy, A.
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caesarsaladinn · 5 months
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if anyone needs me I'll be gazing lovingly at the immunofluorescence pictures I took last night
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a professor activated me this week by talking about lyme disease immunofluorescence and how the CDC has a fucking bullshit criteria of “It’s not Lyme unless five bands fluoresce” and I literally could not stop myself from yelling about how I got a clear as day Lyme read on a single band from a desiccated tick when I was a little kid. i got PRESSED i was so mad
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covidsafehotties · 12 days
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Abstract
Evidence suggests associations between COVID-19 patients or vaccines and glycometabolic dysfunction and an even higher risk of the occurrence of diabetes. Herein, we retrospectively analyzed pancreatic lesions in autopsy tissues from 67 SARS-CoV-2 infected non-human primates (NHPs) models and 121 vaccinated and infected NHPs from 2020 to 2023 and COVID-19 patients. Multi-label immunofluorescence revealed direct infection of both exocrine and endocrine pancreatic cells by the virus in NHPs and humans. Minor and limited phenotypic and histopathological changes were observed in adult models. Systemic proteomics and metabolomics results indicated metabolic disorders, mainly enriched in insulin resistance pathways, in infected adult NHPs, along with elevated fasting C-peptide and C-peptide/glucose ratio levels. Furthermore, in elder COVID-19 NHPs, SARS-CoV-2 infection causes loss of beta (β) cells and lower expressed-insulin in situ characterized by islet amyloidosis and necrosis, activation of α-SMA and aggravated fibrosis consisting of lower collagen in serum, an increase of pancreatic inflammation and stress markers, ICAM-1 and G3BP1, along with more severe glycometabolic dysfunction. In contrast, vaccination maintained glucose homeostasis by activating insulin receptor α and insulin receptor β. Overall, the cumulative risk of diabetes post-COVID-19 is closely tied to age, suggesting more attention should be paid to blood sugar management in elderly COVID-19 patients.
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