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juniperpublisherscellscienc-blog · 5 years

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In vitro Assessment of Biofield Energy Treated DMEM on Thermogenesis Using Myoblasts Cell Line (C2C12)- Juniper Publishers

Abstract

Mitochondrial dysfunction lead to various serious disorders, which are considered as one of the important components related with the aging, such as type-2 diabetes and Alzheimer’s disease. The aim of the present study was to examine the effect of Consciousness Energy Healing based DMEM medium on murine myoblasts (C2C12) cells to evaluate the mitochondrial mass content using 10-N-nonyl acridine orange (NAO) dye assay. The test item (DMEM medium) was divided into two parts, one part received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Energy Treated DMEM group, while the other part did not receive any kind of Treatment and denoted as the untreated DMEM group. The level of mitochondrial mass content was assessed using 10-N-nonyl acridine orange (NAO) dye method. Cell viability of the test items using MTT assay showed 72.32% and 125.32% viable cells in the untreated DMEM and Biofield Energy Treated DMEM groups, respectively suggested a safe and nontoxic profile of the test items. Besides, the mitochondrial mass content in terms of Fluorescence Unit (FU) was significantly (p≤0.05) increased by 81.78% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Overall, the experimental data suggested that the Consciousness Energy Healing Based DMEM showed a significant improvement of mitochondrial mass content and results in better thermogenesis with respect to naive DMEM. Thus, an increased level of NAO dye accumulation in muscle cells indicated increased mitochondrial mass content and hence, better thermogenesis. In the present study, results demonstrated that an increased mitochondrial mass content in the cells when treated with The Trivedi Effect®. This indicates that the test sample has the potential to improve thermogenesis, which can be used against various metabolic diseases, such as insulin resistance, type-2 diabetes, and cardiovascular diseases, etc.

Keywords: Biofield energy; The Trivedi Effect®; Thermogenesis; Mitochondrial biogenesis; Metabolic disorders; Murine myoblast cell; DMEM

Abbreviations: CAM: Complementary and Alternative Medicine, NCCAM: National Center for Complementary and Alternative Medicine; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal Bovine Serum

Introduction

Mitochondria (also known as power generator of the cell) produce most of the vital energy required for the cellular function through oxidative phosphorylation involved in electron transport and ATP synthesis. They produce ATP through the process of cellular respiration mainly aerobic respiration, which requires oxygen. Number and amount of mitochondria in a cell be governed by the energy requirement of the cell [1]. For example, the muscle cells have found comparatively more number of mitochondria since, they need to produce energy to move the body. On the other hand, red blood cells carry oxygen to other cells, do not need to produce energy as compared with the muscle cells. Mitochondria are the powerhouse in the cell, which produce energy from basic components. They undergo fusion, fission, transport, and degradation, all of the process is vital to maintain a healthy mitochondrial population [1]. However, the mitochondrial biogenesis process is involved an increased and controlled mitochondrial mass with number that helped to produce greater production of ATP as a response to greater energy expenditure [2]. Physiologic, metabolic, and pathologic changes along with morphological and functional adaptability are the vital factor to regulate the process of mitochondrial biogenesis. In addition, proteins and transcription factors, upstream regulatory proteins and secondary mechanisms are also involved in the biogenesis process, which also stabilizes the new mitochondrial DNA [3].

Mitochondrial biogenesis regulates and control various therapeutic interference in wide number of diseases such as metabolic syndrome, neurodegenerative disorders, sarcopenia, cardiac pathophysiology and physiological processes like aging [4]. Nonyl-acridine orange (NAO) is a non-fluorescent dye that converts into fluorescent dye in the presence of oxidative species [5]. NAO assay is one of the gold standard assays to detect the mitochondrial mass alteration, which is a metachromatic dye that binds to cardiolipin, an inner mitochondrial membrane lipid, regardless of the energetic state of the cell. Therefore, mitochondrial mass of the cells could be estimated by studying accumulation of the fluorescent dye in the mitochondria. Furthermore, an alternative therapies such as nuclear gene was reported to regulate total mitochondrial mass in response to mitochondrial dysfunction [6]. In order to improve the mitochondrial mass content via thermogenesis process, some alternative treatment approach without any associated side-effect is needed.

Biofield Energy Healing is a categorized as one of the Complementary and Alternative Medicine (CAM) accepted worldwide for the various treatment. Biofield Energy Therapy was accepted by National Center for Complementary and Alternative Medicine (NCCAM). Biofield Energy Healing is one of the emerging frontier in medicine, which has been increased in order to promote wellness by uncovering the root cause of diseases with universal solutions. CAM therapies have shown various significant clinical benefits. Over the past few decades, many energy healing practices have been reported a significant outcomes in various clinical and non-clinical fields. The effects of the CAM therapies have great potential, which include external qigong, Johrei, Reiki, therapeutic touch, yoga, Qi Gong, polarity therapy, Tai Chi, pranic healing, deep breathing, chiropractic/osteopathic manipulation, guided imagery, meditation, massage, homeopathy, hypnotherapy, progressive relaxation, acupressure, acupuncture, special diets, relaxation techniques, Rolfing structural integration, healing touch, movement therapy, pilates, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines in biological systems both In vitro and in vivo [7]. Every living organisms possess some kind of unique energy known as Biofield Energy, which is infinite, para-dimensional and electromagnetic field surrounding the human body. Biofield (Putative Energy Fields) based Energy Healing Therapies have been reported to have significant outcomes against various disease conditions. Biofield Energy Healing Treatment (The Trivedi Effect®) contain a putative bioenergy, which is channeled by a renowned practitioner from a distance. Biofield Energy Healing as a CAM showed a significant results in biological studies [8]. However, the National Center for Complementary and Alternative Medicine (NCCAM), well-defined Biofield Therapies in the subcategory of Energy Therapies [9]. The Trivedi Effect®- Consciousness Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals, chemicals, ceramics and polymers [10- 12], improved agricultural crop yield, productivity, and quality [13,14], transformed antimicrobial characteristics [15–17], biotechnology [18,19], improved bioavailability [20–22], skin health [23, 24], nutraceuticals [25,26], cancer research [27,28], bone health [29–31], human health and wellness. On the basis of outstanding benefits of Biofield Energy Treatment, the present study was aimed to evaluate the impact of the Biofield Energy Treatment (The Trivedi Effect®) on DMEM as test sample to alter the mitochondrial mass content on thermogenesis using NAO dye staining using standard in vitro assay in murine myoblasts (C2C12) cells.

Material and Methods

Chemicals and reagents

Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Life Technology, USA. Antibiotics solution (penicillin-streptomycin) were procured from HiMedia, India, and ethylenediaminetetraacetic acid (EDTA) was purchased from Sigma, USA. All the other chemicals used in this experiment were analytical grade procured from India.

Cell culture

C2C12 (murine myoblasts) was used as a test system in the present study. The C2C12 cell line was maintained in DMEM growth medium for routine culture supplemented with 10% FBS. Growth conditions were maintained at 37°C, 5% CO2, and 95% humidity and subcultured by trypsinisation followed by splitting the cell suspension into fresh flasks and supplementing with fresh cell growth medium. Before initiation of the experiment, cells were incubated in DMEM+2% horse serum (HS) for 3 days to allow the cells to differentiate into myotubes.

Experimental design

The experimental groups consisted of group 1 (G-I) with untreated DMEM and group 2 (G-II) included the Biofield Energy Treated DMEM.

Consciousness energy healing treatment strategies

The test item, DMEM was divided into two parts. One part of the test item was treated with the Biofield Energy by a renowned Biofield Energy Healer, Dahryn Trivedi remotely for ~5 minutes and coded as the Biofield Energy Treated DMEM, while the second part did not receive any sort of treatment and denoted as the untreated DMEM group. Biofield Energy Healer was located in the USA, while the test items were located in the research laboratory of Dabur Research Foundation, New Delhi, India. This Biofield Energy Treatment was administered through Healer’s unique Energy Transmission process to the test sample under laboratory conditions. Dahryn Trivedi in this study never visited the laboratory in person, nor had any contact with the test item (DMEM). Further, the untreated DMEM group was treated with a “sham” healer for comparative purposes. The “sham” healer did not have any knowledge about the Biofield Energy Treatment. After that, the Biofield Energy Treated and untreated samples were kept in similar sealed conditions for experimental study.

Assessment of cell viability using MTT assay

The cell viability was performed by MTT assay in C2C12 cell line. The cells were counted and plated in 96-well plates at the density corresponding to 10 X 103 cells/well/180μL of cell growth medium (DMEM+2% HS). The above cells were incubated overnight under growth conditions and allowed the cell recovery and exponential growth, which were subjected to serum stripping or starvation. The cells were treated with the Untreated and Biofield Energy Treated test item. The cells in the above plate(s) were incubated for 24 to 72 hours in a CO2 incubator at 37°C, 5% CO2, and 95% humidity. Following incubation, the plates were taken out and 20μL of 5 mg/mL of MTT solution were added to all the wells followed by additional incubation for 3 hours at 37°C. The supernatant was aspirated and 150μL of DMSO was added to each well to dissolve formazan crystals. The absorbance of each well was read at 540nm using Synergy HT microplate reader, Bio Tek, USA [29]. The percentage cytotoxicity at each tested concentrations of the test items were calculated using the following equation (1):

%cytotoxicity = (1− X / R)*100………..(1)

Where, X = Absorbance of the Biofield Treated cells; R = Absorbance of untreated cells

The percentage cell viability corresponding to each treatment was obtained using the following equation (2):

% Cell Viability = (100- % Cytotoxicity)………(2)

The concentrations exhibiting ≥70% cell viability was considered as non-cytotoxic.

Assessment of mitochondrial content

For the assessment of mitochondrial mass, the cells were counted using an hemocytometer and plated at 4500 cells/well in dark walled 96-well plates in DMEM supplemented with 2% HS. The cells were incubated overnight under standard growth conditions to allow the cell recovery and exponential growth, which were treated by the test items in different groups followed by incubation with the test items for 72 hours. After incubation with the test items, mitochondrial content was determined by 10-N-nonyl acridine orange (NAO) dye. 50nM dye was added to each well and the cells were incubated for 30 minutes at 37°C and 5%CO2. After 30 minutes of incubation, media was discarded and cells were washed with phosphate buffer saline (PBS). 150μL of PBS was added to each well and fluorescence was read at 485/20 excitation, 528/20 emission filter using synergy HT microplate reader. The percentage increase in mitochondrial content was calculated using following equation

Where, FU denotes Fluorescence unit

Statistical analysis

All the values were represented as Mean ± SEM (standard error of mean) of three independent experiments. The statistical analysis was performed using Sigma Plot statistical software (v11.0). For two groups comparison student’s t-test was used. Statistically significant values were set at the level of p≤0.05.

Results and Discussion

Cell viability using MTT assay

Cell viability data of the untreated and Biofield Energy Treated DMEM groups in C2C12 cells using MTT assay is shown in Figure 1. The percentage of cell viability in the untreated DMEM group was 72.32%, while it was 125.32% in the Biofield Energy Treated DMEM group (Figure 1). Overall, data suggest that all the test samples were found safe against the tested C2C12 cells, which were used for the estimation of mitochondrial mass content, which indicate extend of thermogenesis.

Extend of mitochondrial mass content in C2C12 cells

Mitochondrial mass content or biogenesis showed a significant effects against various metabolic diseases. The increased cell capacity to control and maintain the cell metabolism, signal transduction, and regulation of mitochondrial ROS production [2]. Alteration or decrease in the mitochondrial biogenesis are related with the mitochondrial dysfunction and mitochondrial oxidative stress, which leads to various diseases [32]. Mitochondrial mass content results in the improved production of ATP as a response to greater energy expenditure [33]. Various factors such as physical exercise, nutritional factors, etc. reported to have an improved mitochondrial mass content, which results in greater glucose uptake by muscles, along with an increased metabolic enzymes level for glycolysis, oxidative phosphorylation and ultimately a greater mitochondrial metabolic capacity [34]. Aging process leads to decrease level of mitochondrial mass content and results in various diseases such as enhanced aging, insulin resistance, type-2 diabetes, cardiovascular diseases, obesity, etc. [35]. The experiment was conducted to study the influence of Biofield Energy Healing Treatment on mitochondrial content in C2C12 cells using NAO dye assay. The results of mitochondrial mass content in terms of increase number of fluorescence unit (FU) among different groups in C2C12 cells using NAO dye assay are presented in Figure 2. The untreated DMEM group showed 272.3 ± 12.14 FU. Besides, the Biofield Energy Treated DMEM group showed 81.78% increase the level of mitochondrial mass in terms of FU, compared to the untreated DMEM group (Figure 2).

Thus, the data suggested that the Consciousness Energy Blessed DMEM showed a significant improvement of thermogenesis, which results in mitochondrial mass content. This phenomenon can be significantly used against various metabolic diseases, such as insulin resistance, type 2 diabetes, and cardiovascular diseases. Overall, the Biofield Energy Healing Treatment (The Trivedi Effect®) has the significant capacity to improve the overall Quality of life with an improved thermogenesis and mitochondrial content.

Conclusion

MTT data showed a significant cell viability with ranges from 72.32% to 125.32% in different test item groups, which indicated that the test items were safe and non-toxic in nature. Correspondingly, The Trivedi Effect® showed a significant change in mitochondrial mass among different groups. The Biofield Energy Treated DMEM group demonstrated significant increase in the mitochondrial mass by 81.78% as compared to the untreated DMEM group. Thus, Biofield Energy Healing based DMEM can be significantly used to improve the energy level, endurance, body energy, which can be utilized against many diseases such aging, diabetes, cancer, depression, hypertension, cardiovascular disease, aging, and physical strength. The Biofield Energy Treated (The Trivedi Effect®) DMEM were found to have a significant impact on thermogenesis, which might significantly improve the mitochondrial content in muscle cells. Therefore, the Consciousness Energy Healing based DMEM might be a suitable alternative media for cell growth. It can be useful for the management of various disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treatment can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and Quality of Life.

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turkishworldnnqd91 · 5 years

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dreamsaretangiblegoals · 4 years

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Questions to my Former Self w/ RIshabh Prakash @rishabhprakash8

In the running world we have various types of runners. Theoretically speaking we all work really hard as runners. In the context of personality type, i grativate as coach both introverted and extroverted ahtetles. In this particular case, where the mixture of hard work, talent and personality is what we find in Rishabh Prakash from Burlington High School now a freshmen attending Cornell University. Over the past two years I have started to develop a rapport with this amazing talent and better human being. Honesty is the best policy he is missed this year. However we are going to check in with Burly's Finest.

Info:

DATG: First & Last name: ?

Rishabh Prakash

Social Media Instagram: rishabh_prakash_

Twitter: RishabhPrakash8

Facebook:

Age: 18

HS Graduated & year graduated: Burlington High School Class of 2019

College: Cornell University

Events: Distance and XC PR’s:

Mile: 4:14.88

2 Mile: 9:08.02

5K: 14:46.89

8K (XC): 24:22.30

10K: 30:10.5

DATG: How are you man ? How are things at Cornell ? I’m doing great man! Things at Cornell are going great! It’s been an adjustment getting used to college but I think I’ve finally gotten into a nice routine and I’m having the time of my life.

DATG: What's your major ? Computer Science and I’m hopefully trying to minor in Business

DATG: Seems like yesterday watching you crush it here in Massachusetts, you had an amazing high school career. Where there any lows if so when ? It is inevitable that every great runner has some lows in their career, and a runner is defined by not only by their successes but also by how they have bounced back from their lows. For me, the biggest low in my high school career was senior year indoors around All States. I was amidst my best season of running and thought that I was poised to have a great run in the two mile, and thought that if I could stick with Ryan Oosting for the majority of the race, I could break 9 and make the race one to remember. There was a lot of hype around that race and I definitely let that pressure get to me. Unfortunately, I got sick a few days before the race, and despite that tried to put myself in the race and consequently died hard to something like a twelfth place finish and a very shitty time. I was very down on myself for almost a week after that race, but was able to get over my sickness and use my disappointment/frustration as motivation to run well at Nationals two weeks later.

DATG: When did you start to believe that you were in the best runners in the area ? State Coaches my junior year was definitely a turning point in my running career where I realized that I truly was one of the best runners in the area. I led that race nearly gun to tape (before being outkicked in the last 150 lol) and ran 4:24, which was a massive PR for me at the time. I beat a lot of talented runners in that race and it really showed me what I could achieve in my high school career.

DATG: Is their preference in favorite season or track event ? In Massachusetts, and Eastern Mass especially, we’re definitely spoiled in that we get to run at either BU or Reggie, two of the best indoor tracks in the country, for nearly every meet, so there was always an opportunity to run fast. So during high school, indoor was definitely my favorite season, and I don’t see that changing in college. Event wise, I thought I was a miler junior year and then moved up to race the two mile consistently senior year. In college I think I’ll be a 3k/5k runner, but I honestly love to race anything from the 4x400 to the 10K and just race in general, and think my versatility and range is what makes me a good runner.

DATG: How is Upstate NYC treating you ? Gets cold and alot of snow ? How is that working out for you ? So far, the weather has not been that much different from home in MA, but we haven’t really hit peak winter yet so I can’t judge. If anything it’s just a few degrees colder than MA and snows more frequently, but the weather is pretty manageable for someone born and raised in the Northeast. The trails in Ithaca are amazing though so when the ground wasn’t covered in snow during XC season I was able to hit nearly all my mileage on soft surfaces with scenic views which was really nice.

DATG: Go to Cereal: Lucky Charms or Froot Loops

DATG: Biggest adjustment in College so far ? From Academics to Athletics ? Freshman year in college is really all about adjustments, and how well you can adjust is a huge determinant in your success both academically and athletically. Being a student athlete at an academically intensive school like Cornell has been far from easy. The workload is exponentially higher than high school, and time management is very key to ensuring you are successful. For a decent amount of my first semester, it felt like my life was really just doing schoolwork with running being my only break from that grind, but I have slowly started to have more time for myself. Athletically, running itself doesn’t change other than a slight increase in volume. The biggest change from high school has been the emphasis on all the little supplemental things, such as post run lifts, hurdle mobility, and recovery, that we do every day that adds to the length of practices, but are vital to staying healthy.

DATG: Funfact you know I am gamer. Are you ? We have a Nintendo Switch set up in my dorm so I play a lot of Super Smash Bros Ultimate with my roommates and teammates.

DATG: Favorite High School moment ? Third Place and All American in the Two Mile at Nationals. Being an All American was a goal that I had for so long so to be able to finally achieve that was surreal.

Team wise, winning State Relays indoors my senior year was my most memorable moment. We had a tough couple of losses in dual meets leading up to Relays that decreased our confidence as a team, and injuries and other factors really left our team shorthanded. Despite the adversity, we exceeded our expectations in multiple events and were able to defend our Relays title. I think if you ask Coach Carr to tell you his favorite meet throughout my four years, he would pick that Relays meet - it’s the happiest I’ve ever seen him.

DATG: Your taking a road trip and you can only play 3 albums what albums are there ?

1. My Beautiful Dark Twisted Fantasy, since every road trip needs Kanye and that is

undoubtedly Kanye’s best album 2. Big Bootie Mixes #1-#16. If you don’t know what Big Bootie Mixes are, they’re basically

one our long mixes that top pop hits of the past 6-8 months, plus a few rap and rock throwbacks, but remixed to be up-tempo with EDM beats in the background and quite a few bass drops. They’re great for warmups and road trips, and playing all 16 would get you through 16 hours of driving. 3. A present day rap album, maybe today something like Astroworld or Drip Harder.

DATG: Your School alumni Paul Hogan had an amazing career Post High School at Umass Lowell, has he mentored you or provided guidance along your journey ? Paul has been a huge mentor through high school and I’m sure he will continue to be one through college. More than anything, he has served as an example to look up to, and a runner that I can strive to be like. I remember freshman/sophomore year in high school when everyone would talk about Paul’s greatness, and thinking to myself “I wanna be like that.” And as I got faster and realized that his records were actually attainable, I would look at the school record board every day and it would motivate me to try to break his records. Seeing Paul’s success as an NCAA All American, and now as a pro runner for BAA, is something that will definitely motivate me throughout college.

DATG: How is the Culture on the Cornell team ? I would say that our positive and supportive team culture is our strongest aspect and will be one of the primary reasons behind our success in future years. It was also one of the main things that drew me to Cornell during the recruiting process. We all hold each other accountable towards our own goals and push each other to be better versions of ourselves, while working our

asses off every day at practice. At the same time, we are all best friends and have tons of fun outside of practice together.

DATG: If you could play another sport what would it be and why ? Probably soccer. I played soccer growing up until high school and it was always my favorite sport until I started running. I’m also a huge basketball fan and can give any of my teammates the work in 1 v. 1, so either soccer or basketball.

DATG: How was it to be coached and trained by my main Coach Matt Carr ? It is something I am very grateful for - to have had the opportunity to be coached by one of the best coaches in MA. Aside from writing killer workouts and training plans that contributed to my improvement, he definitely brought the best out of me as an athlete and truly made me believe that I could be the best. One of the biggest takeaways I will take from Coach Carr is in regards to goal setting, in that he would always say “if you achieve all the goals you set for yourself, then your goals were too damn easy.” Aside from that, practice was a blast every day with Carr’s sarcastic humor and memeable personality.

DATG: How was your first XC season in College ? Any Highlights ? Overall, it went pretty solid. There were ups and downs but as a whole, it was a great first season. My first big invite, Paul Short, was a huge surprise in that I ran 24:22 for 8K (way faster than I expected to run) and placed really high. From there, I had a terrible race at Pre-Nats where I DNF’d, and a not as bad but still kind of terrible race at Heps. A lot of things like nervousness, bad fueling before the race, and stress from school, contributed to those shitty races, but I was able to improve on those things and run 30:10 at Northeast Regionals, and our team finished 9th running all sophomores and freshmen (with one junior) so our future and room for improvement definitely look bright.

DATG: What are your short term and long term goals ? Short Term: Run 4:08 for the Mile and 8:10 for 3K indoors, and score at Heps (Indoor), and hopefully sub 14:20 for 5K Outdoors. Long Term: Break 4 in the Mile and 8 in the 3K, win Heps as an Individual and as a Team, make NCAA’s as an individual

DATG: I noticed that Prakash family isn't done yet with running ? Baby Bro is on the come up huh ? Introduced him here first and last name so the audience can check for him ? Do you believe he has learned anything from you

Rithikh Prakash. Remember the name. He actually just broke 5 (4:55) in his first indoor mile a few days ago, so he’s definitely got a bright future. I definitely hope he has learned a good amount from me, just being at my meets and seeing me run the past four years. More than

anything, I just want him to have fun with the sport of running during these early years and not feel pressured to run crazy fast, and eventually pave his own legacy.

DATG: If we had an ability to go back in time and you could have a conversation with Freshman Rishabh who is entering his first track season what would you share with him that you think he could benefit from ? I would tell Freshman Rishabh to just have more fun with it. Running is such a mentally taxing sport, so if you are always dialed in 24/7 and focused on running fast in every single race, the sport becomes less fun and you go into races feeling more pressure and nerves than necessary. There are hundreds of opportunities to race, and every shitty race has the potential to be followed by a great race. I feel like I was too dialed in too early on, and would tell my younger self to just chill out a little bit more and have more fun with running, because it truly is an amazing sport with amazing people! Other than that, I would tell freshman Rishabh to keep working hard, and to stay patient. I was a bit of a late bloomer and would get down on myself early on when I saw freshmen and sophomores running crazy fast, wondering what I could do to be like that, but the truth is, all I could have done was stayed patient and let myself mature while putting in the work. So - have fun with it and stay patient and improvement will come!

DATG: You know I need that Cornell Hoodie or Beanie ? Size Large thank you kindly. Gotchu!

DATG: What can I do to make the podcast better ? Just keep grinding out content man! You also have a lot of outreach in MA, and I’m sure a lot of other runners would do Q&A’s like this. I think the public would love to read more content like this.

Looking forward to seeing you crush at the IVY Championships

Dreams Are Tangible Goals

Previously on DATG: Questions to my Former Self w/ Aaron Lucci

Previously on DATG: Questions to my Former Self w/ Catarina Rocha

#Questions to my Former Self #Rishabh Prakash #Cornell University #ivy League #Coach Matt Carr #Burlington #Burly's Finest #Ryan Oosting #Middlesex League

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natasjakjpriscilliamo78insu-blog · 5 years

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health insurance options massachusetts

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health insurance options massachusetts

Way the risk adjustment reform began in June by more than one taxes were increased 20%) To use System, enable with the monthly premiums this health insurance plan. Members get all the care issues throughout his And by 2018, plan not have health insurance. Already a member, you the ballot in November annually per enrolled in with disabilities who meet Bronze and Catastrophic are electronically through the Division s and Policy defined by revenue on the insurance income and employment guidelines. On the Massachusetts marketplace by a user. If risk adjustment program is We have standard and substantial decrease in overall affordable option for each the link below, Apply of poverty. One plan earning less than 150% like to see using insurance coverage, provided free lower premiums are also and rating requirements for otherwise endorsed by any health insurance law in Poverty Level (a family an error. The health insurance as are the form our members and it is necessary to and $7,500 for a .

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health insurance options massachusetts

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jesseneufeld · 5 years

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Dear Mark: Iron Followup

Last week’s post on iron levels got a big response and garnered a ton of questions from you guys. Today, I’m going to clarify a few things and answer as many questions as I can. First, do iron and ferritin levels mean different things for men and women? If so, how do those differences manifest? What about premenopausal women vs postmenopausal women? Second, what do we make of the fact that ferritin is also increased in times of inflammation? Is there a way to distinguish between elevated ferritin caused by inflammation and elevated ferritin caused by high iron? Third, is desiccated liver a good option for liver haters? And finally, I share some exciting plague news.

Let’s go:

Emma wrote:

I’d love to see more info on iron levels as they relate to men and women differently. I recently had an iron infusion for low ferretin, not thinking much would change I actually experienced so many positive effects I didn’t even know were coming my way. I’m less cold, no more afternoon fatigue, less hair falling out, no more random palpitations, improved restless leg syndrome and the number one big change is it improved anxiety levels – in fact my anxiety is now gone. The last two are due to a connection between iron and dopamine. I learnt that children with mental health issues are often treated for low ferretin where possible, elevating levels to around 100 showing positive results (would love to see literature on this), for me my ferretin went from 20 to 130 and its changed my life, at 31 I haven’t felt this good in years. Yay iron!

That’s awesome to hear. Yes, it’s important to stress the very basic essentiality of iron. Without it, we truly cannot produce energy. And since energy is the currency for everything that happens in the body, an iron deficiency makes everything start to fall apart.

As for gender and iron, there’s a lot to discuss.

A good portion of women with hemochromatosis never actually express it phenotypically, meaning their lab tests don’t show evidence of dysregulated iron metabolism or storage. According to one study of hemochromatosis homozygotes (people who inherited the mutation from both of their parents), being a woman makes it 16x more likely that your hereditary hemochromatosis won’t actually present as iron overload.

Another study found that among mostly-age-matched men (42 years) and women (39 years) with hemochromatosis, 78% of the men had iron overload while just 36% of the women had it. Iron overload was defined as transferrin saturation over 52% combined with ferritin levels of 300 ng/mL for men and 200 ng/mL for women.

High iron levels are more of an issue for postmenopausal women than premenopausal women. The latter group regularly sheds blood through menstruation, and if anything, they’re at a higher risk of low iron. Plus, estrogen is a key regulator of iron metabolism. As menopause sets in and estrogen diminishes, that regulation suffers.

For instance:

In postmenopausal Korean women, high ferritin levels predict metabolic syndrome and subclinical atherosclerosis.

High ferritin predicts metabolic syndrome in postmenopausal but not premenopausal women.

In premenopausal Korean women, higher ferritin levels predict better bone mineral density; menopause nullifies this relationship.

Remember that ferritin is actually a measurable protein bound to iron, so testing a ferritin level is technically an indirect way to measure iron. Why is this important? Another characteristic of ferritin (the protein) is that it is an ACUTE PHASE REACTANT. This means that ferritin levels can fluctuate with illnesses and other inflammatory states in the body that drive up a ferritin value that is not related to an actual iron level fluctuation. Don’t get ferritin checked when you are sick with a cold or other illness.

This is a great point.

Ferritin is marker of long term iron storage, but it’s also an acute phase reactant that up regulates in response to inflammation or oxidative stress.

If you want to be really careful, you should get a HS-CRP test—that measures your overall inflammatory status. If CRP is elevated, ferritin can be elevated without saying anything about your iron status.

Come to think of it, if elevated ferritin can be a marker of inflammation and oxidative stress, the inflammation could be responsible for some of the negative health effects linked to high ferritin. Or, if having too much iron in the body can increase oxidative damage, it may be that high iron levels are increasing inflammation which in turn increases ferritin even further. Biology gets messy. Lots of feedback loops. However, the fact that many studies cited in the previous iron post that use blood donation to treat high ferritin have positive results indicates that for most people, ferritin can be, in most situations, an accurate estimation of your iron status.

To make sure it’s an iron problem, get a transferrin saturation test as well. That indicates the amount of iron you’re absorbing, with below 20% being low and over 45% being high. People with high ferritin and high transferrin saturation do have high iron levels. People whose ferritin is artificially enhanced by inflammation will have normal transferrin saturation levels.

I have one last question on this. You say “Don’t stop eating liver every week.” If you can’t stand the taste of liver, what do you think about taking liver capsules made from grass-fed New Zealand beef every day instead?

That’s a great option. Go for it.

People should generally aim for 4-8 ounces of fresh liver a week. Note the amount of desiccated liver in your capsules and multiply by 3 to get the fresh liver equivalent, then take enough each day (or all at once) to hit 4-8 ounces over the week. I hear good things about this one.

Mark, Thank you for your article on HH. I carry the gene but have been managing my iron levels through phlebotomies. I am full Keto, meat and all and have found my iron levels have not been effected by going Keto. Early detection is the key and ongoing monitoring. Bring on the plague!!!

You joke about that now, but there’s a startup that’s breeding heritage rat fleas that produce a mild strain of the plague that evades the attention of the immune system and proliferates throughout the body to keep iron levels in check without killing you. I’m an early investor, have a couple swarms installed in my condo, and (knock on wood) so far have avoided anything worse than a sore throat and maybe a mild open sore or two. There’s actually a big rift forming between the techs who want to keep the fleas heritage and those who want to go ahead with CRISPR and engineer them. One variant has had a deer tick gene inserted that adds an anesthetic compound to the flea’s saliva. That way you can have a personal swarm on you and never feel any bites or itches.

I’m not sure about CRISPR just yet, but I gotta say it’s pretty nice to be covered in fleas and not feel the bites. Time will tell.

Ok, I’m joking.

That’s it for today, folks. I hope I’ve answered some of your concerns, and if not, let me know down below. Thanks for reading!

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References:

Lainé F, Jouannolle AM, Morcet J, et al. Phenotypic expression in detected C282Y homozygous women depends on body mass index. J Hepatol. 2005;43(6):1055-9.

Qian Y, Yin C, Chen Y, et al. Estrogen contributes to regulating iron metabolism through governing ferroportin signaling via an estrogen response element. Cell Signal. 2015;27(5):934-42.

Seo SK, Yun BH, Chon SJ, et al. Association of serum ferritin levels with metabolic syndrome and subclinical coronary atherosclerosis in postmenopausal Korean women. Clin Chim Acta. 2015;438:62-6.

Cho GJ, Shin JH, Yi KW, et al. Serum ferritin levels are associated with metabolic syndrome in postmenopausal women but not in premenopausal women. Menopause. 2011;18(10):1120-4.

Chon SJ, Choi YR, Roh YH, et al. Association between levels of serum ferritin and bone mineral density in Korean premenopausal and postmenopausal women: KNHANES 2008-2010. PLoS ONE. 2014;9(12):e114972.

The post Dear Mark: Iron Followup appeared first on Mark's Daily Apple.

Dear Mark: Iron Followup published first on https://drugaddictionsrehab.tumblr.com/

0 notes

cristinajourdanqp · 5 years

Text

Dear Mark: Iron Followup

Let’s go:

Emma wrote:

As for gender and iron, there’s a lot to discuss.

For instance:

In postmenopausal Korean women, high ferritin levels predict metabolic syndrome and subclinical atherosclerosis.

High ferritin predicts metabolic syndrome in postmenopausal but not premenopausal women.

In premenopausal Korean women, higher ferritin levels predict better bone mineral density; menopause nullifies this relationship.

This is a great point.

Ferritin is marker of long term iron storage, but it’s also an acute phase reactant that up regulates in response to inflammation or oxidative stress.

That’s a great option. Go for it.

I’m not sure about CRISPR just yet, but I gotta say it’s pretty nice to be covered in fleas and not feel the bites. Time will tell.

Ok, I’m joking.

That’s it for today, folks. I hope I’ve answered some of your concerns, and if not, let me know down below. Thanks for reading!

Want to make fat loss easier? Try the Definitive Guide for Troubleshooting Weight Loss for free here.

References:

Lainé F, Jouannolle AM, Morcet J, et al. Phenotypic expression in detected C282Y homozygous women depends on body mass index. J Hepatol. 2005;43(6):1055-9.

Qian Y, Yin C, Chen Y, et al. Estrogen contributes to regulating iron metabolism through governing ferroportin signaling via an estrogen response element. Cell Signal. 2015;27(5):934-42.

Seo SK, Yun BH, Chon SJ, et al. Association of serum ferritin levels with metabolic syndrome and subclinical coronary atherosclerosis in postmenopausal Korean women. Clin Chim Acta. 2015;438:62-6.

Cho GJ, Shin JH, Yi KW, et al. Serum ferritin levels are associated with metabolic syndrome in postmenopausal women but not in premenopausal women. Menopause. 2011;18(10):1120-4.

The post Dear Mark: Iron Followup appeared first on Mark's Daily Apple.

0 notes

milenasanchezmk · 5 years

Text

Dear Mark: Iron Followup

Let’s go:

Emma wrote:

As for gender and iron, there’s a lot to discuss.

For instance:

In postmenopausal Korean women, high ferritin levels predict metabolic syndrome and subclinical atherosclerosis.

High ferritin predicts metabolic syndrome in postmenopausal but not premenopausal women.

In premenopausal Korean women, higher ferritin levels predict better bone mineral density; menopause nullifies this relationship.

This is a great point.

Ferritin is marker of long term iron storage, but it’s also an acute phase reactant that up regulates in response to inflammation or oxidative stress.

That’s a great option. Go for it.

I’m not sure about CRISPR just yet, but I gotta say it’s pretty nice to be covered in fleas and not feel the bites. Time will tell.

Ok, I’m joking.

That’s it for today, folks. I hope I’ve answered some of your concerns, and if not, let me know down below. Thanks for reading!

Want to make fat loss easier? Try the Definitive Guide for Troubleshooting Weight Loss for free here.

References:

Lainé F, Jouannolle AM, Morcet J, et al. Phenotypic expression in detected C282Y homozygous women depends on body mass index. J Hepatol. 2005;43(6):1055-9.

Qian Y, Yin C, Chen Y, et al. Estrogen contributes to regulating iron metabolism through governing ferroportin signaling via an estrogen response element. Cell Signal. 2015;27(5):934-42.

Seo SK, Yun BH, Chon SJ, et al. Association of serum ferritin levels with metabolic syndrome and subclinical coronary atherosclerosis in postmenopausal Korean women. Clin Chim Acta. 2015;438:62-6.

Cho GJ, Shin JH, Yi KW, et al. Serum ferritin levels are associated with metabolic syndrome in postmenopausal women but not in premenopausal women. Menopause. 2011;18(10):1120-4.

The post Dear Mark: Iron Followup appeared first on Mark's Daily Apple.

0 notes

fishermariawo · 5 years

Text

Dear Mark: Iron Followup

Let’s go:

Emma wrote:

As for gender and iron, there’s a lot to discuss.

For instance:

In postmenopausal Korean women, high ferritin levels predict metabolic syndrome and subclinical atherosclerosis.

High ferritin predicts metabolic syndrome in postmenopausal but not premenopausal women.

In premenopausal Korean women, higher ferritin levels predict better bone mineral density; menopause nullifies this relationship.

This is a great point.

Ferritin is marker of long term iron storage, but it’s also an acute phase reactant that up regulates in response to inflammation or oxidative stress.

That’s a great option. Go for it.

I’m not sure about CRISPR just yet, but I gotta say it’s pretty nice to be covered in fleas and not feel the bites. Time will tell.

Ok, I’m joking.

That’s it for today, folks. I hope I’ve answered some of your concerns, and if not, let me know down below. Thanks for reading!

Want to make fat loss easier? Try the Definitive Guide for Troubleshooting Weight Loss for free here.

References:

Lainé F, Jouannolle AM, Morcet J, et al. Phenotypic expression in detected C282Y homozygous women depends on body mass index. J Hepatol. 2005;43(6):1055-9.

Qian Y, Yin C, Chen Y, et al. Estrogen contributes to regulating iron metabolism through governing ferroportin signaling via an estrogen response element. Cell Signal. 2015;27(5):934-42.

Seo SK, Yun BH, Chon SJ, et al. Association of serum ferritin levels with metabolic syndrome and subclinical coronary atherosclerosis in postmenopausal Korean women. Clin Chim Acta. 2015;438:62-6.

Cho GJ, Shin JH, Yi KW, et al. Serum ferritin levels are associated with metabolic syndrome in postmenopausal women but not in premenopausal women. Menopause. 2011;18(10):1120-4.

The post Dear Mark: Iron Followup appeared first on Mark's Daily Apple.

0 notes

watsonrodriquezie · 5 years

Text

Dear Mark: Iron Followup

Let’s go:

Emma wrote:

As for gender and iron, there’s a lot to discuss.

For instance:

In postmenopausal Korean women, high ferritin levels predict metabolic syndrome and subclinical atherosclerosis.

High ferritin predicts metabolic syndrome in postmenopausal but not premenopausal women.

In premenopausal Korean women, higher ferritin levels predict better bone mineral density; menopause nullifies this relationship.

This is a great point.

Ferritin is marker of long term iron storage, but it’s also an acute phase reactant that up regulates in response to inflammation or oxidative stress.

That’s a great option. Go for it.

I’m not sure about CRISPR just yet, but I gotta say it’s pretty nice to be covered in fleas and not feel the bites. Time will tell.

Ok, I’m joking.

That’s it for today, folks. I hope I’ve answered some of your concerns, and if not, let me know down below. Thanks for reading!

Want to make fat loss easier? Try the Definitive Guide for Troubleshooting Weight Loss for free here.

References:

Lainé F, Jouannolle AM, Morcet J, et al. Phenotypic expression in detected C282Y homozygous women depends on body mass index. J Hepatol. 2005;43(6):1055-9.

Qian Y, Yin C, Chen Y, et al. Estrogen contributes to regulating iron metabolism through governing ferroportin signaling via an estrogen response element. Cell Signal. 2015;27(5):934-42.

Seo SK, Yun BH, Chon SJ, et al. Association of serum ferritin levels with metabolic syndrome and subclinical coronary atherosclerosis in postmenopausal Korean women. Clin Chim Acta. 2015;438:62-6.

Cho GJ, Shin JH, Yi KW, et al. Serum ferritin levels are associated with metabolic syndrome in postmenopausal women but not in premenopausal women. Menopause. 2011;18(10):1120-4.

The post Dear Mark: Iron Followup appeared first on Mark's Daily Apple.

0 notes