#caco

[whewchilly]

Team 55 via Calum

#they stripped him down … #carlos sainz jr #australian gp 2024 #caco #pedro #ricky #pierluigi

[justc2world]

Rupert : It would've been a f boring race if it wasn't for Carlos Sainz

FACTS✨️

#took the words out of my mouth #rupert manwaring #caco #carlos sainz jr #carlos sainz #scuderia ferrari #f1 #monza gp 2023

[ehcahache]

If I didn't know how Carlos looks like, I would think Caco was the one who actually won yesterday, look at them 😭

#carlos sainz jr #caco #singapore gp 2023

[artemispt]

The annual pic on the stairs

#no Oli and her clones #no Ana and Blanca #maybe that’s why Carlos is with that face 😂 #carlos sainz #carlos sainz jr #carlos sainz sr #caco #Reyes Vazquez de Castro #winter break 2023 #sainz family #Sainz family Christmas pic

[nmcartoons]

"Forever and ever and ever and ever!"

#my art #digital art #art #artists on tumblr #fanart #phisnom #phisart #stupidbutterfly #caco #uhyeah #pastra #ultim8nik #toxic cesspit #fnaf #five nights at freddys #freddy and friends

[lies]

Sometimes when I'm birdwatching

#sometimes when i’m birdwatching #birds #caco #zion national park #it's cool to be able to read the wing tags #and learn the birds' histories #this dude is j3 #he was soaring with his son #1k #1k has the distinction #of having been the 1000th condor #entered in the breeding program records #from 22 remaining birds #to now #more than 30 years later #three different populations #flying free #still critically endangered #perpetually on the brink #of winking out forever #lead poisoning #avian flu spread by poultry flocks #j3's mate died of avian flu this year #but he's been soaring with another bird #a young female #and the volunteer who was kind enough to share with us #says he hopes they'll continue nesting at this site

[landopics]

#lando norris #golfing as always #carlando #Carlos sainz jr #Carlos sainz sr #caco

[aksh369]

Pierre hard launching Carlos and Rebecca 😭😭

#he deleted it lmao #pierre gasly #carlos sainz jr #carlos sainz #f1 #rebecca donaldson #caco #Rupert #such dorks I love them #😭😭😭😭😭😭

[insilanar]

Poor Caco 😭

He is so done with Lando & Carlos... 😂🤣

#carlos onoro #caco #carlando #f1 #'I finally have time to get over jet lag' #'instead I'm stuck with these crazy lovebirds' #I love him 🤗

[whewchilly]

carlosonoros Today has officially been our last day on track with Dr. Manwaring (a.k.a @.p1_coach ) as part of Team55!

Impossible to summarize in one post all the good times we’ve had together during the past 7 years (I’ve literally spend the last 2 hours laughing on my own going through all the pictures and videos I have in my phone!)

It’s been an absolute pleasure and an honor to work alongside you through thick and thin my friend. I truly wish you all the best for the future!

Once Team55, always Team55! 😁

#❤️❤️❤️ #bye Rupert :’) #caco #abu dhabi 2023

[justc2world]

PAUSE ‼️

#the fact that caco is there #Sr got his game face on #carlos sainz #carlos sainz sr #caco #carlos onoros #carlos sainz jr #f1 #formula 1 #mercedes

[ehcahache]

Team 55 walking in on style

#carlos sainz jr #team 55 #rupert manwaring #caco #abu dhabi gp 2023

[thegentlemancollects]

#CaCo #Blonde #Dress

[artemispt]

Good morning to me…. 🫠

#and they had to put the logo RIGHT ON TOP 🥲 #the V #armporn #rear end #the back of his neck… his hair #Carlos’ back compilation #carlos sainz #carlos sainz jr #caco #canada gp 2023 #shiga sports japan

[smores-artist]

These were pretty fun art style experiments of the 'Phisnom crew' as well as a few guests! I also was very satisfied with my interpretation of Peyo's art style for Clyde, so it got to be colored in unlike the others.

#pastra #gobzone #caco #nou #uhyeah #phisart #art style experiment #artists on tumblr

[axispharm]

Caco-2 Permeability Assay

In the development of small molecule drugs, it is necessary to evaluate the bioavailability of the compound as early as possible, so as to lay the foundation for subsequent development. In addition to using experimental animals to evaluate bioavailability, various in vitro analysis methods have been developed to evaluate the ability of compounds to penetrate the intestinal wall. These in vitro experiments are not only lower in cost but also higher in throughput and shorter experimental time. Among them, Caco-2 monolayer cell permeability analysis assay has become one of the standards in vitro methods.

CaCO-2 cells are derived from human colon cancer and have the typical characteristics of intestinal epithelial cells, such as the formation of a polarized monolayer columnar epithelium, and densely differentiated microvilli on the top surface of the cells neatly arranged to form a striated border and intercellular connection.

The determination of Caco-2 cell permeability not only helps to determine intestinal permeability but also can identify specific transporters or efflux proteins and intestinal phase II drug-metabolizing enzymes. Therefore, this Caco-2 permeability assay provides valuable information for drug development in an effective and reproducible manner, which is not available in non-biological models, and even in many other biological models.

The Efflux Ratio can be calculated by measuring the transport from A-B and B-A in two different directions, so as to determine whether a molecule has active efflux.

Caco-2 Permeability Assay Protocol

Compound required

10 mM DMSO 100µL or 2 mg

Compound Test Concentration

10µM

Cell Culture

21 days

Replicates

N=2

Incubation Conditions

Incubation in CO2 incubator at 37ºC for 90 min

Positive control compound

digoxin （or other compounds）

Integrity of the monolayer

TEER>200 Ω•cm2

Analysis method

LC-MS／MS

Data delivery

Papp, Efflux Ratio, %Recovery

Data calculation

Papp = CRecx VRec/ (A x t x C0) Efflux Ratio = Papp (B→A) /Papp (A→B) Recovery = [(Vrec x Crec) + (Vd x Cd)] / (Vd x C0)

#caco #2 #permeability #assay