The orange did indeed prove out! I've separated the f2 generation into their het and homo groups. I lost the paper I was keeping count on, but I remember the ratio being consistent with my 50/50 expectation.
Here they are! My camera really wanted to enhance the orange. The video still on the right is more accurate.
Their coloration is noticeably grainy and looks darker than regular 'orange' to me, but I might be imagining things. There's not too much variation.
Dark Orange X Spanish Orange
Now HERE'S where it gets interesting! The 'Dark Orange' X 'Spanish Orange' cross was a little test breeding meant to verify whether the same recessive amelanism gene was responsible for the orange color in both morphs. I expected to see either all grey (meaning they were separate genes) or all orange (meaning they were the same). What I didn't expect to see was three distinct phenotypes.
There's the classic orange that I expected, a browner/darker orange, and three greys for some reason.
So YES! The classic recessive amel gene is responsible for the orange in 'dark orange'.
But did the 'dark' gene just prove out? Is it a dominant or co-dominant allele? Idk! I have to wait until the homozygous f2s are bigger to see if all or any of them share this trait. Their having grainy coloration and being mixed ages makes it frustratingly difficult to tell at this stage.
I attribute the even coloration of this test cross to the 'Spanish Orange' side of the family, since pure Spanish Orange stock is known for its smooth coloration.
Now to address the greys. Yeah, I don't know what happened here. The mother has been isolated with the progenitor in a harem setup since way before she was sexually mature. The only thing I can think is that one of the f2 sons managed to breed with her before I removed them. I'm not sure how that's possible as I removed them when they were absolutely tiny but it's the most likely explanation I can think of. I've separated the three greys into their own bin for now. Maybe the answers will be further down the road.
Unfortunately, I can't find the original dark orange male who started all this. I'm not sure if he passed away or if he somehow eluded me when I sifted. Wherever he is... o7
Porcellio scaber “Rose Quartz” being simply exquisite. I love how dainty and expressive isopod antennae are—they show exactly what he is focusing on and where he wants to go.
so far he and his probable brother (now deceased) are the only two of these, but hope his little kids carry the mutation and can breed more
I've finally started to remove isopods from the nature center's colony so they don't get overcrowded and I am finding some really cool ones. Look at this one!!
More macros, you can see her egg pouch in the first couple ones! Isopods carry their babies in a pouch until they're ready to leave. See how many babies you can count! (Hint: the newest ones are pure white and look an awful lot like springtails, look for a uniform oval shape!)