#t25flask

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Application of cell culture flasks in culturing Sf9 cells

Sf9 cells are Spodoptera frugiperda cells, which are usually used to produce various medicinal proteins and viruses, or for basic research. When culturing sf9 cells, the commonly used cell culture consumables are cell culture flasks.

Sf9 cells are derived from the Insect Pathology Laboratory of the United States Department of Agriculture and are derived from the IPLBSF-21 cell line. The cells are regular with particles and can be cultured in suspension or adherent culture. This cell has a high expression level (especially intracellular proteins), has a faster growth rate, effective protein folding, glycosylation is similar to mammalian cells, and relatively easy enzymatic deglycosylation (for protein structure determination) Favorable), is widely used in the expression of V LPs.

#fudau #fdcell #t25flask #cellcultureflasks #sf9cell

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The difference between the cell culture flask Sealed Cap and the Vent Cap

Cell culture flasks are mainly used for adherent cell culture, and cell culture scale-up in laboratory or industrial production processes. According to the different culture conditions, this cell culture container has two types of lids: Sealed Cap and Vent Cap. So, what is the difference between these two types of caps

           FuDau T25 Cell Culture Flasks

The sealing cap is completely sealed and is suitable for cell and tissue culture under sealed conditions, so that the culture environment is completely isolated from the outside world. The breathable cover has a hydrophobic filter membrane with a pore size of 0.22um, which can meet the needs of gas exchange in cell and tissue culture, and can effectively prevent cross-contamination. It is suitable for open culture conditions.

The two types of caps for cell culture flasks are first used in different environments. The vent cap is suitable for use in an environment with carbon dioxide, while the sealed cap is mainly used in a culture environment without carbon dioxide. Secondly, there is no ventilation hole on the surface of the sealing cover, and there is no filter membrane. If ventilation is required, the cover needs to be unscrewed by a quarter of a turn, while the vent cover has a filter membrane, and there are ventilation holes on the top of the cover, which can meet the needs of cell culture. The need for gas exchange.

         FuDau T75 Cell Culture Flasks

On the whole, the difference between the cell culture bottle sealing cap and the vent cap is mainly reflected in the application scenario and whether it has a filter membrane. No matter what kind of cap it is, it is to adapt to different cell culture needs, and it also reflects the different functions of cell culture containers. 

#fudau #fdcell #cellcultureflasks #t25flask #t75flask

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T25 Flask - FuDau

Fudau T25 flask use polystyrene (PS) raw materials, ultra-vacuum plasma surface modification(TC treated) process to enhance the adherence performance of the cells, providing more conducive growth environment to the cells .

#fudau #fdcell #T25flask #t25