Wolverine from X-Men: Evolution was a gay man, or at the very least queer. My evidence is as follows:
No defining/memorable romantic relationships with women AT ALL during the show's run. Jean was a teenager, he was an adult, and - unlike the Ultimate X-Men comics which served as inspiration for this series - these two did not have a romantic relationship nor did they have any romantic tension, the relationship between them instead mirroring a brother/sister one. The same can be said about Logan's other high-profile comics romantic interest, Storm, where their relationship was more like siblings as well rather than anything with romantic undertones.