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Human or the environment microbial sequencing can be used for a variety of applications, such as pathogen detection and environmental monitoring.
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Proteomics is the analysis of all protein components expressed in a genome or a cell, tissue, or organ. Among them, the study of subcellular proteomics is particularly important, because it not only reduces the complexity of proteome but also can indicate the location and functional information of proteins, the most important of which is the study of mitochondrial proteomics.
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Microbial isolation and cultivation refer to the process of microorganism separating, purifying and cultivating in different media and culture conditions based on the traits and growth characteristics of microorganisms.
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In vivo/in vitro assessment of mitochondrial function can be a useful tool for mechanistic studies of liver diseases, as well as for the evaluation of (novel) therapeutic interventions.
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Fungal Genome Editing
Fungal DNA sequences with unknown functions inspire efforts in the identification of these genes and their biological characteristics, which have become a research hotspot in the post-genomic era.
Creative Biogene can use CRISPR/Cas9 gene editing, T-DNA insertion, homologous recombination, RNA interference and other technologies to assist scientific researchers in exploring gene functions and modifying fungal genomes.
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Microbial Genome Editing
With the development of modern molecular biology technology, there are many new methods of microbial breeding with more directional and positive mutation, which greatly promotes the accuracy and efficiency of microbial genome editing.
1. Microbial genome editing based on homologous recombination uses its own Rec system to carry out homologous recombination of exogenous DNA to achieve allelic replacement of target genes, and recombination of genetic information between two homologous strands of DNA.
2. CRISPR/Cas9 method for microbial genome editing (including gene knockout, site-directed mutation, site-directed insertion of foreign sequences, etc.). Compared with traditional gene knockout methods, this method is more convenient for subsequent experimental research.
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