Smelly’s Song Of The Week…..
XTC - Making Plans for Nigel
TOS…..I was going to post another track from the new Idles album but @loveaxiomatic beat me to it. In an interview about the album last week they talked about cover versions, and this is a track they liked to do at their early gigs…..
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poggers circuit fighter
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Common uses of bioinformatics
💡Sequence analysis
Analyzing DNA and protein sequences to identify genes, regulatory regions & mutations.
💡Gene expression
Analyzing RNA expression data from experiments like microarrays or RNA-seq to understand gene regulation.
💡Phylogenetics
Constructing evolutionary relationships between organisms based on genetic data and genomic comparisons.
💡Molecular modeling
Predicting protein structure and docking drugs to proteins using computational modeling and simulation.
💡Databases & Data mining
Developing databases like GenBank to store biological data and mining it to find patterns.
💡Genomics
Studying entire genomes, including sequencing and assembling genomes as well as identifying genes and genomic variations.
Follow @everythingaboutbiotech for useful posts.
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My altar for Pagan Community Retreat in Hemet, CA.
Instagram: madisonvanderlinde
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Samples are fucked up in a way that could not possibly be human error and is obviously environmental.
Me: I did this. I brought shame on this lab. Somehow, some way, this was me. I am a bad nugget :c
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Polymerase chain reaction reaction???????
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Barnacle cusp
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hwang hyunjin is work of art
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When you start 2 separate PCR reactions an hour apart but they finish within 60 seconds of each other
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Smelly’s Song Of The Week…..
Queens of the Stone Age - No One Knows
TOS 🤷🏼♂️
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Chain Termination PCR
-- also called Sanger sequencing
-- used to determine the nucleotide sequence of DNA
-- manual or automated
-- PCR occurs first
-- DNA is denatured to break the double-strand into two single strands
-- primers anneal to each strand
-- they extend to create two double strands
-- in Sanger sequencing / chain termination, ddNTPs are added along with dNTPs
-- ddNTPs can be added randomly as the chain extends
-- they stop the chain
-- this is because they can’t form phosphodiester bonds like dNTPs can
-- next, the products are separated by size
-- this is done with gel electrophoresis
-- DNA polymerase works in the 5′ to 3′ direction
-- each terminal ddNTP will correspond to a specific nucleotide in the original sequence
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Sometimes I doodle cute shit because I can.
Haruka and Majima would totally cheer for Kiryu as he beats the snot out of a ten-year old in Pocket Ciruit racing.
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Managed to get a clean PCR and electrophoresis result (lanes 2 and 3) for my plasmidless EAEC. Top bar with the red in lane 4 is the pure plasmid, lining up with the plasmid DNA in the ladder. The bacterium without the plasmid will make a nice negative control for finding an EAEC fimbriae stain. Not sure what the bars at the bottom past the ladder are though.
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Its terrifyingly true...
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