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#clinical microbiology
cbirt · 5 months
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The introduction of Full Laboratory Automations has served to revolutionize the workflows of labs worldwide, helping with the automation of routine tasks and the capacity for digital analysis of the results. However, a significant limitation is the inability to identify and interpret bacterial cultures, a task that is considered essential in the field of microbiology and diagnostics. A group of researchers from the University of Brescia seeks to remedy this with the introduction of a new deep-learning model, “DeepColony,” that can help with culture interpretation and quantification.
Despite the introduction of several new techniques in microbiology labs all over the world, the practice of bacterial culture and interpretation is still the primary method for diagnosing the presence of infectious pathogens. There are many reasons for its popularity, simplicity, and cost efficiency being prime among them. However, despite all of the advantages it offers, the interpretation of cultures accurately still remains a challenging task, prone to human error and constrained by the availability of qualified and experienced staff. Though other tasks requiring visual interpretation, such as cytology and hematology, have since been automated through the use of machine learning algorithms, bacterial culture remains unchanged for the most part.
Considering this lack of resources, the creation and development of Full Laboratory Automation systems have allowed for the automation and digitization of various steps involved in culturing, such as processing, streaking, and incubation, at the end of which images are uploaded for analysis. This has helped with the reduction of turn-around times, better efficiency and quality, as well as lower costs. However, the interpretation of these images still remains a significant challenge. While prior image analysis tools have been created for certain specific scenarios, these aren’t as helpful for other circumstances. The need for a generalized image analysis tool capable of consistent interpretations is great.
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koursweetyhtf · 12 days
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tmmediaindia · 5 months
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Understand the Spiderman of the Microverse: XLD Agar
Learning XLD’s X, Y, and Z can be challenging, but not with us. In the previous article, we discussed the various needs and applications of XLD (click on the link if you haven’t read it). In this, we’ll talk more about its composition, principle, observations, and modifications.
What is the composition of XLD Agar?
It was developed by Welton Taylor in 1965. XLD stands for Xylose Lysine Deoxycholate Agar and is a bright pink or red-colored solid opalescent gel medium. Knowing its key contents will help you better understand its principles and differentiating abilities.
Ingredients g/l
Yeast Extract 3 g
L-lysine 5 g
Xylose 3.75 g
Lactose 7.5 g
Sucrose 7.5 g
Sodium Deoxycholate 1 g
Sodium Chloride 5 g
Sodium Thiosulfate 6.8 g
Ferric Ammonium Citrate 0.8 g
Phenol red 0.08 g
Agar 12.5 g
Distilled water 1 Litre
Yeast extract provides the medium with nutrients, vitamins, peptides, and other essential growth factors. Xylose, lactose, and sucrose are rich sources of fermentable carbohydrates. For the detection of fermentation of these carbs in the medium, a pH indicator, phenol red, is added. The addition of xylose to the medium accounts for the differentiation of various fermenting enteric bacteria from species of Shigella that do not ferment it. Most enteric pathogens, including Salmonella,can ferment xylose, which results in the formation of acidic byproducts and a change in the color of the medium from pink to yellow, whereas Shigella colonies remain red. In some cases, Salmonella imitates Shigella colonies and gives red colonies, which are then differentiated by the production of Hydrogen sulfide gas by the metabolism of thiosulfate and give black centers. Enterobacteria such as E. coli can ferment lactose in the medium.
XLD Agar’s composition can be adjusted according to one’s needs and choices and is hence available in many different variations, but the basic principle remains the same.
Observation and Inferences on XLD Agar:
Observation Inferences
Red colonies, some with black centers Salmonella spp.
Red colonies Shigella spp.
Yellow to orange colonies Coliforms
Pink, flat, and rough colonies Pseudomonas aeruginosa
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While learning about XLD Agar, a question that must have run through your thoughts would’ve been, “Where is XLD Agar available?” Let’s look into it.
Where is XLD Agar available?
XLD Agar is available on the market as Dehydrated Culture Media in a number of variations according to one’s requirements, including plant-based and animal-based options.
These Dehydrated Culture Media can be dissolved in distilled water according to the concentration mentioned on the package, autoclaved, poured, and used.
TM Media, the microbiology division of Titan Biotech Ltd., offers a wide range of XLD Agar as Dehydrated Culture Media in a number of variations suitable for all your needs, operations, and specific requirements.
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The product range includes the following:
XLD AGAR MODIFIED (as per ISO) TM 1621: XLD Agar, modified, is a selective and differential medium for the isolation of gram-negative enteric pathogens from clinical specimens or food products. It is a modification of the original formulation of Taylor that allows selective isolation of Salmonella typhi, E. coli, Salmonella enteritidis, Salmonella typhimurium, and Shigella flexneri. It is recommended by the ISO committee, and the composition and performance criteria of this medium are as per the specifications laid down in ISO 6579-1:2017.
XLD AGAR (VEG.) TMV 492: Xylose Lysine Deoxycholate Agar (Veg) is prepared by replacing Sodium Deoxycholate with synthetic detergent No.III, which makes the medium free of BSE/TSE risks. Xylose Lysine Deoxycholate Agar (Veg) is suitable for the isolation and identification of enteric pathogens from stool samples.
XLD AGAR (as per USP/EP/JP/BP) TMH 112: This medium is employed for pharmaceutical testing and non-sterile product testing for the detection of Salmonella after enrichment in Rappaport VassialidiasSalmonella Enrichment Broth in accordance with the harmonized method of USP/EP/BP/JP/IP.
XLD AGAR TM 1448: It is used for pharmaceutical testing and nonsterile product testing for the detection (or absence) of Salmonella after enrichment in Rappaport Vassialidias Salmonella Enrichment Broth in accordance with IP.
XLD AGAR TM 492: XLD Agar exhibits increased selectivity and sensitivity as compared to other plating media, e.g., SS Agar, EMB Agar, and Bismuth Sulphite Agar. The media formulation does not allow the overgrowth of other organisms over Salmonella and Shigella. Samples suspected of containing enteric pathogens, along with other mixed flora, are initially enriched in Modified Semisolid RV Medium Base.
Why choose TM Media’s XLD Agar?
TM Media’s superior-quality and diverse Microbiological Culture Media are certified by ISO, CE, GMP, FSSAI, and FSSC.
TM Media provides unrivaled convenience, dependability, and efficacy, as well as versatility and flexibility, by manufacturing a contamination-free medium with a longer shelf life and reproducible outcomes.
Conclusion:
XLD Agar is a selective and differential agar medium primarily used for the isolation and differentiation of different enteropathogenic organisms that are well-known to cause diseases like Food poisoning, Gastroenteritis, and other Digestive illnesses.
By choosing TM Media, consumers invest in precision, quality, and excellence. TM Media's product portfolio includes over 2000 Dehydrated Culture Media, along with Ready-to-Use Culture Media, Biological Media Bases, Media Supplements, Lab Chemicals, and many more.
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heighpubsseo · 1 year
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Growth and Herbage Yield of Gamba (Andropogon Gayanus, Kunth) and Kyasuwa (Pennisetum Pedicellatum, Tan) Grasses in Sokoto Semi-Arid Zone of Nigeria
Abstract
This paper reports on the growth and herbage yield of Andropogon gayanusand Pennisetum pedicellatumin the Sokoto Semi-arid zone of Nigeria, with a view to identifying most productive among the species for forage feed production in the study area. Field experiment was conducted during the 2010 rainy season at the Dabagi teaching and research farm of Usmanu Danfodiyo University, Sokoto, Nigeria. Parameters evaluated include plant height, number of leaves, leaf length, leaf width, number of tillers per plant and herbage dry matter yield at 10 WAS. The data generated was analyzed using the Statistical Analysis System in a Randomized Complete Block Design. Fisher’s Least Significance Difference (LSDt<0.05) test was used for mean separation. Results showed that, P. pedicellatumproduced significantly (P<0.05) taller plants (110.01cm versus 77.25cm), higher (P<0.05) number of leaves per plant (6.39 versus 4.00) and higher (P<0.05) herbage dry matter yield (3.518 t DM ha-1 versus 2.037 t DM ha-1) compared to the A. gayanus. A. gayanusrecorded longer (P<0.05) leaves (46.83cm versus 27.90cm) and higher (P<0.05) number of tillers per plant (70.61 versus 28.67). Leaf width for the two grass species were statistically (P>0.05) similar (1.75cm versus 1.42cm) at 10 WAS. Merit analysis showed that P. pedicellatumrecorded a higher score of 9 points and was ranked 1st while A. gayanusscored 8 points and was ranked 2nd. It may be concluded that both the P. pedicellatumand A. gayanusshowed potentials for high productivity, thus; both species are recommended for production in the study area.
Read more about this article: https://lupinepublishers.com/biotechnology-microbiology/fulltext/growth-and-herbage-yield-of-gamba-andropogon-gayanus-kunth-and-kyasuwa.ID.000127.php
Read more Lupine publishers Goggle Scholar Articles: https://scholar.google.com/citations?view_op=view_citation&hl=en&user=X4tPijcAAAAJ&cstart=20&pagesize=80&citation_for_view=X4tPijcAAAAJ:35N4QoGY0k4C
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snailboxes · 10 months
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pathparazzi · 1 year
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Lactophenol cotton blue - Aspergillus versicolor. Don't ask me how I know this, this was speciated well after I took the picture. It is recognizably, however, Aspergillus.
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k151k1ll1ll4 · 11 months
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local lab rat
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burikumu · 1 year
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Week Recap (Nov. 27 - Dec. 3, 2022)
Part 1
Beginning of Microbiology section
Tried to study for the week (Except on thursday siniksik ba naman yung lab sa finals week edi sana nagrereview kami diba)
Crammed the rest of the subjects the night before finals
Didn't finish reading and woke up with a headache during finals
Already on the acceptance stage even if the grades are not out yet
┈┈┈┈․° ☣ °․┈┈┈┈
Part 2
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xenomorphone · 2 years
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My friend has been visiting for a few days to look at possible vet clinics to work at around here and it was so nice having someone here to do things with. And also someone on my level. We talked about parasitic worms at dinner and it was ok!!!!! We looked at pics of osteosarcomas!!! I need these kind of people in my life
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varamreprogenesis · 20 days
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Courses in Clinical Embryology - Varam Reprogenesis
Embark on Your Journey in Clinical Embryology: Explore Courses from Varam Reprogenesis
Are you passionate about the field of assisted reproductive technology (ART) and eager to specialize in clinical embryology? Varam Reprogenesis offers a range of comprehensive courses in clinical embryology designed to provide aspiring embryologists and fertility specialists with the knowledge, skills, and hands-on experience needed to excel in this dynamic and rewarding field.
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Why Choose Courses in Clinical Embryology from Varam Reprogenesis?
Expert Faculty: Our courses are taught by a team of renowned experts and experienced practitioners in the field of clinical embryology. With their vast knowledge and practical insights, our faculty members provide invaluable guidance and mentorship to help you navigate the intricacies of clinical embryology.
Comprehensive Curriculum: Our courses cover a wide range of topics related to clinical embryology including gamete handling, embryo culture, cryopreservation techniques, quality control measures, and laboratory management. The curriculum is carefully designed to provide a comprehensive understanding of the theoretical principles and practical applications of clinical embryology.
Hands-On Training: We believe in learning by doing. Our courses include hands-on training sessions conducted in state-of-the-art embryology laboratories equipped with the latest ART equipment and technology. Participants have the opportunity to gain practical experience in performing essential embryology procedures under the guidance of experienced instructors.
Flexible Learning Options: Whether you're a novice seeking to kickstart your career in clinical embryology or a seasoned practitioner looking to enhance your skills, we offer courses tailored to meet your specific learning needs and career goals. Choose from a variety of course formats, including in-person workshops, online modules, and blended learning options.
Career Advancement: Completing a course in clinical embryology from Varam Reprogenesis can open doors to exciting career opportunities in fertility clinics, ART laboratories, research institutions, and academic settings. Our courses are recognized and respected within the industry, providing you with a competitive edge in the job market.
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Embark on Your Journey in Clinical Embryology with Varam Reprogenesis:
Whether you're looking to start a rewarding career in clinical embryology or advance your skills and expertise in the field, Varam Reprogenesis offers the perfect platform to pursue your goals. Explore our range of courses in clinical embryology and take the first step towards a fulfilling and successful career in assisted reproduction. Contact us today to learn more about our courses and start your journey with Varam Reprogenesis.
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Located in the heart of Thrissur, Hygea Med Laboratories is a leading Medical Laboratory in Thrissur that offers top-notch and comprehensive diagnostic services. With a team of highly skilled professionals and state-of-the-art equipment, Hygea Med Laboratories is dedicated to providing the best and quality services to the residents of Thrissur.
When it comes to healthcare, accuracy and reliability are of utmost importance. That's where Hygea Med Laboratories stands out. The laboratory is equipped with the latest technology and follows strict quality control measures to ensure accurate and precise test results every time. This not only helps in accurate diagnosis but also aids in better treatment and management of diseases.
Hygea Med Laboratories offers a wide range of diagnostic services including blood tests, urine tests, imaging services, and more. The laboratory is equipped with advanced equipment and machines, which are regularly maintained and calibrated by a team of trained technicians. This ensures that the results obtained are accurate and reliable.
One of the key factors that make Hygea Med Laboratories stand out from other medical laboratories in Thrissur is their team of highly skilled professionals. The laboratory has a team of experienced and qualified pathologists, technicians, and support staff who are well-trained in their respective fields. They are dedicated to providing patients with the best care and ensuring that all tests are conducted with utmost accuracy and efficiency.
At Hygea Med Laboratories, patient comfort and convenience are given top priority. The laboratory has a spacious waiting area and comfortable sample collection rooms to ensure a stress-free experience for patients. The staff is friendly and approachable, making the entire process of getting tests done hassle-free.
Apart from providing quality diagnostic services, Hygea Med Laboratories also offers home sample collection services. This is especially beneficial for elderly patients or those who are unable to visit the laboratory. The samples are collected by trained phlebotomists and are transported to the laboratory with utmost care and efficiency.
In addition to its top-notch services, Hygea Med Laboratories also maintains a strict code of ethics and confidentiality. Patient information and test results are kept confidential and are only shared with authorized individuals. This ensures that patients feel comfortable and secure while getting their tests done.
Overall, Hygea Med Laboratories is the go-to medical laboratory in Thrissur for accurate and reliable diagnostic services. The laboratory's commitment to quality, advanced technology, and skilled professionals make it the best choice for all your healthcare needs. So, the next time you need to get a test done, head to Hygea Med Laboratories for a hassle-free and superior experience.
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toolbox201 · 1 month
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Microbiology
Welcome to Trulife Diagnostics Centre, your trusted destination for comprehensive healthcare services in Hyderabad. Our “Microbiology” department is at the forefront of precision diagnostics and patient care.
Why Choose Trulife Diagnostics Centre for Microbiology Testing?
Cutting-Edge Technology: We pride ourselves on staying at the forefront of microbiology diagnostics. Our advanced equipment and techniques ensure the highest level of accuracy and reliability in our tests.
Expert Microbiologists: Our team of expert microbiologists, technologists, and pathologists is dedicated to providing timely and accurate results. We’re committed to the well-being of our patients.
Wide Range of Tests: Trulife Diagnostics Centre offers an extensive range of microbiology tests, covering areas such as bacterial and viral cultures, antibiotic sensitivity testing, and more.
Rapid Reporting: We understand that timely results are crucial for effective patient care. Rest assured that our reporting process is designed for efficiency. Stringent Quality Control: Our quality control measures are rigorous, ensuring that every microbiology report is reliable and precise.
Patient-Focused Approach: Your comfort and satisfaction are our priorities. Our compassionate staff and patient-friendly environment make your experience hassle-free.
Best Diagnostics Centre in Hyderabad
Trulife Diagnostics Centre has earned a well-deserved reputation as the best diagnostics centre in Hyderabad. Our commitment to excellence and patient-centered care sets us apart.
Our Microbiology services exemplify our dedication to offering reliable and accurate diagnostic solutions. Whether you require routine culture and sensitivity testing or specialized microbiology services, our department is fully equipped to meet your needs.
When it comes to precise, timely, and patient-centric microbiology testing, make Trulife Diagnostics Centre your first choice. We are the best diagnostics centre in Hyderabad, dedicated to serving your health needs.
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immentallyunstableus · 4 months
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Studying evil microbiology for my evil microbiolgy exam from the evil microbiology faculty
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tipsywench · 6 months
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planning my schedule for next semester and I'm gonna fucking die lol
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gratisolsblog · 6 months
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