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draconicmaw · 7 months
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Easy and Simple way to extract DNA from Strawberries
Just taught my nephews how to extract DNA from strawberries, and it's really easy, so I'll tell you all, too
You'll need:
2 strawberries (fresh or frozen works but frozen is preferred)
A resealable plastic bag
1/2 cup of water
2 tsp dish soap
1 tsp salt
Isopropyl (rubbing) alcohol (~70% is preferable)
2 cups
Coffee filters
First things first -- sample prep. If your strawberries have their greens attached, pluck them off. Give em a good rinse, toss them in the bag, and make sure you seal that thing tight or there will be strawberry guts everywhere. Mash gently with your fist. Grinding your knuckles in helps, or you can be like my nephew and use the flat edge of a butter knife to mash them.
Okay, let's get our "DNA extraction liquid" prepped. Put the half cup of water, tsp of salt, and 2 tsp of dish detergent in a cup. Stir gently. Try not to make too many bubbles. I mixed everything else first and then gently submerged my dish detergent into solution and swirled until homogenous.
NOICE. Prep is done. Take 2 tsps of your super duper cool extraction liquid and put it in your mashed strawberry bag. Reseal it again, and mash gently. Try not to make too many bubbles! (Sad, I know.)
Cool, great, your strawberries should be mushy and goopy by now. Press your coffee filters (I recommend 3 or 4 if you have thinner filters) into the cup and fold their edges over the rim. Pour your mashed strawberry in. Let it sit for a minute for the liquid to sleep through. When you think it won't drop anymore or you just get impatient, you can try to squeeze the rest out by hand.
You should have some fragrant strawberry extract at your disposal! What you want to do now is gently pour the rubbing alcohol down the side of the cup so that it doesn't disturb the surface too much. About equal volume to your extract.
WARNING: at no point after adding your extraction liquid should you consume the mashed strawberries. Especially after addition of isopropyl alcohol, which will give you "gastric disturbances" (aka diarrhea)
Bam. That's all you have to do. Because you'll start noticing little bubbles floating up... Except those aren't bubbles! They are long, white strands of DNA!
Now how did this all work...?
Well, in step one, you mashed up your strawberries, and that helped expose and separate the cells in the strawberry. Your "DNA extraction liquid" is a detergent. Detergents have a polar and a nonpolar side on each molecule! This allows it to drag nonpolar things (like fats and other lipids) into solution! This is why we use detergents to clean dishes and clothing. Cells use a lipid membrane, which we dissolved with the detergent! Also, it binds to ionic (charge carrying) substances. Guess what is ionic... Your DNA! DNA has a strongly negative backbone. It bound itself to the DNA and made it less polar.
So, when you poured the isopropyl alcohol in (a weakly polar solvent), the detergent-coated DNA preferentially diffused to a less polar solvent!
So that goopy, stringy substance you collected is DNA. If you REALLY wanted to and had the means to, you could collect the DNA (a popsicle stick works really well), wash it in 70% ethanol, and do further testing on it!
Anyways, do with this what you will! Have fun!
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draconicmaw · 7 months
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Hello! I'm a big fan of your work on ao3; I've noticed you stopped posting on tumblr/ao3 a while ago, is there any updated social media you still post writing on? Regardless, I hope that life has been treating you well. : D
Hello! I only really post on Ao3 and here. I've been on a bit of an unintentional hiatus, but hopefully I will be able to post something soon. Thank you so much for the ask! I hope life is treating you well, too!
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draconicmaw · 1 year
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is it too much to ask for someone who thinks me a fierce and formidable beast, a force to be reckoned with? to not handle me with care because they think me soft and delicate, but to handle me with care because they think me a sharp blade to be respected?
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draconicmaw · 1 year
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Revoween!
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Hello, everyone! Week-of-Revo and the Revolutionshipping Discord Server would love to invite you to participate in Revoween!
Yes, you are guessing exactly what we mean. A Halloween-themed Revolutionshipping event! 
The event will take place Oct. 29-31 (though late entries are more than welcome). 
The prompts will be:
October 29 – Costume and/or Party
October 30 – Phantom and/or Immortality
October 31 – Creature and/or Full Moon
Rules:
Any sort of content you would like to produce (fic, art, or otherwise) will be welcomed!
Tag @week-of-revo on tumblr posts or add #Revoween.
Mature or Explicit content will be reblogged to @week-of-revo-2 and must be tagged properly.
Have fun!
Lastly, we would like to apologize for the late notice, but we hope you decide to join us in the spirit of the season!
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draconicmaw · 1 year
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[Image ID: a comic-style picture shows two people standing in front of a painting depicting the torment of sinners in hell. Underneath is the quote "If you need the threat of hell to be a good person, then you're just a bad person on a leash". End ID.]
that post thats like "i hope christianity as a global power collapses" and there's just tons of hand-wringing "enjoy living in a lawless wasteland with no morals" comments like. congrats. you are a living illustration of the fucking point.
if your sense of morality is anchored to the bible and you think people would just start killing people etc without it, congrats, your worldview is fucked and you should do some introspection why.
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draconicmaw · 1 year
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I love bad movies that try so hard to be good that they are even worse
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draconicmaw · 1 year
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Omfg I had this same issue with my cat Aruna. She was SO ROTUND and i thought that for some reason her worms were just sticking around (she had all sorts of issues that were taking a while to clear up because she was found emaciated and in a literal dumpster). She was getting a normal amount of food for her size, weight, and age, so I thought the worst.
One day, I hear rustling coming from the pantry.
ARUNA HAD CLIMBED INTO THE BIG CAT FOOD BAG AND WAS EATING TO HER HEART'S CONTENT!!!
Of course she was fat! SHE WAS PILFERING EXTRA RATIONS. (And Sunny would JUST WATCH)
I should have known but this only marked the beginning of her food thievery. What a trash goblin
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shes fat
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draconicmaw · 1 year
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draconicmaw · 1 year
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check out this periodic table
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draconicmaw · 1 year
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Imagining some Han Solo and Chewbacca -esque scenes where Anzu makes her wolf noises and Atem just says "No, you can't do that, you'll scare people" and whether he actually understands what she is saying is totally up to the reader.
Hmmmm also thinking about revolutionshipping on this fine day.
Modern AU where monsters are real and people know about them. Anzu is a werewolf. She's been repressing/managing her transformations with a variety of tranquilizers, among other drugs. But it's causing behavioral changes in her day-to-day life (she's getting more aggressive, very emotionally volatile, etc). So she and her doctor decide it would be best if she did a free-range transformation. Legally, in order for this to be done, she has to have a shift partner who is not a werewolf but just as strong as one to watch over her during her transformation.
So she goes to a local club for the things that go bump in the night to try to get herself a shift partner. Enter Atem, elder vampire, who decides he quite likes this pup. He decides to be her shift partner. Shenanigans ensue.
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draconicmaw · 1 year
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Hmmmm also thinking about revolutionshipping on this fine day.
Modern AU where monsters are real and people know about them. Anzu is a werewolf. She's been repressing/managing her transformations with a variety of tranquilizers, among other drugs. But it's causing behavioral changes in her day-to-day life (she's getting more aggressive, very emotionally volatile, etc). So she and her doctor decide it would be best if she did a free-range transformation. Legally, in order for this to be done, she has to have a shift partner who is not a werewolf but just as strong as one to watch over her during her transformation.
So she goes to a local club for the things that go bump in the night to try to get herself a shift partner. Enter Atem, elder vampire, who decides he quite likes this pup. He decides to be her shift partner. Shenanigans ensue.
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draconicmaw · 1 year
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I am in a rare peachshipping mood. So here is my peachshipping idea.
The Backup Plan
Essentially, after Anzu goes through a bad breakup in their early 20s and Yugi is struggling to find someone who isn't just interested in hooking up, they get drunk together and make a pact that, if they are both single by the time they are 36, they will just get married to each other.
Well, the time comes. They are both single and lonely, and, when drinking together again, remember their pact and decide to go through with it.
After all, it's better to get married to the bff you love than someone you met on a blind date that went horribly wrong...
Neither expect the growing sexual and romantic tension growing between them following the wedding.
Or, the friends-to-spouses-to-lovers peachshipping story absolutely no one asked for
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draconicmaw · 2 years
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Me: I want to get a 100 gallon fish tank, put 1 goldfish in it, and name the fish B.A.G.
Roommate: why BAG?
Me: BIG ASS GOLDFISH
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draconicmaw · 2 years
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JOHN AND EMILY HERE TO TELL YOU THAT CHEMISTRY MATTERS
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draconicmaw · 2 years
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Come join us, Red...
Fandom friends seem to have lots of Fun in discord, maybe I should be active on discord too?? But I'm very Nervous 😂😂😂
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draconicmaw · 2 years
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Not fair. Blindfold the duck, too.
What the duck?
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draconicmaw · 2 years
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one of my beloved friends (very autism) was in.. not denial but ignorance sounds mean. but that abt themself last time you brought up the raads r test so i sent it to the gc (full of autism) and they were like psh. fake test no one could get below 100. they know now but i think about it so much
The thing is. When you take the autism test. And you see your score is in the 100 to 160 range. You think. Oh this is probably the middle? Middle autism. Tinge of autism. Your relatives calling you bright but shy autism. Just a whiff of autism. And then you see the score ranges. And you go. This test is lying to me there is absolutely no way the majority of people score under 65. The 65 number is such a low cutoff and so many of these experiences are clearly universal a score under 65 is something they made up in a lab. People who score under 65 are obviously scoring just under that mark from 59 to 64 and they’re also obviously lying or purposely misrepresenting their experiences as less severe than they are. And then you find out there are real people who get a 20 or 30 or 7 on it. And you go. Ah
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